Cloned (Comment) | Organism |
---|---|
expressed in Aspergillus oryzae strain MS2 | Penicillium citrinum |
Crystallization (Comment) | Organism |
---|---|
modeling of structure at 2.2 A resolution | Penicillium citrinum |
native enzyme and in complexes with the inhibitors 1-deoxymannojirimycin and kifunensine, hanging drop vapor diffusion method, using 17-22% (w/v) polyethylene glycol 6000, 50 mM KH2PO4, pH 4.6 | Penicillium citrinum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-deoxymannojirimycin | modeling of binding to enzyme | Penicillium citrinum | |
kifunensine | modeling of binding to enzyme | Penicillium citrinum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Penicillium citrinum | |
Ca2+ | inhibitors kifunensine and 1-deoxymannojirimycin bind to the essential Ca2+ ion | Penicillium citrinum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Man9GlcNAc2 + H2O | Penicillium citrinum | - |
Man8GlcNAc2 (isomer 8A1,2,3B1,3) + D-mannose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium citrinum | - |
- |
- |
Penicillium citrinum | P31723 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and CM-Toyopearl 650M column chromatography | Penicillium citrinum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Man9GlcNAc2 + H2O | - |
Penicillium citrinum | Man8GlcNAc2 (isomer 8A1,2,3B1,3) + D-mannose | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Penicillium citrinum |
Synonyms | Comment | Organism |
---|---|---|
alpha1,2-mannosidase | - |
Penicillium citrinum |
msdC | - |
Penicillium citrinum |
General Information | Comment | Organism |
---|---|---|
physiological function | comparison of the molecular structure of the Penicillium citrinum Golgi enzyme, and yeast and human endoplasmic reticulum enzymes. There is a greater degree of freedom to bind the oligosaccharide in the active site of the fungal enzyme than in the yeast and human ER alpha1,2-mannosidases | Penicillium citrinum |