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Literature summary for 3.2.1.113 extracted from

  • Lobsanov, Y.D.; Vallee, F.; Imberty, A.; Yoshida, T.; Yip, P.; Herscovics, A.; Howell, P.L.
    Structure of Penicillium citrinum alpha-1,2-mannosidase reveals the basis for differences in specificity of the endoplasmic reticulum and golgi class I enzymes (2002), J. Biol. Chem., 277, 5620-5630 .
    View publication on PubMed
Search for more literature for 3.2.1.113

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Aspergillus oryzae strain MS2 Penicillium citrinum

Crystallization (Commentary)

Crystallization (Comment) Organism
modeling of structure at 2.2 A resolution Penicillium citrinum
native enzyme and in complexes with the inhibitors 1-deoxymannojirimycin and kifunensine, hanging drop vapor diffusion method, using 17-22% (w/v) polyethylene glycol 6000, 50 mM KH2PO4, pH 4.6 Penicillium citrinum

Inhibitors

Inhibitors Comment Organism Structure
1-deoxymannojirimycin modeling of binding to enzyme Penicillium citrinum
kifunensine modeling of binding to enzyme Penicillium citrinum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Penicillium citrinum
Ca2+ inhibitors kifunensine and 1-deoxymannojirimycin bind to the essential Ca2+ ion Penicillium citrinum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Man9GlcNAc2 + H2O Penicillium citrinum
-
 Man8GlcNAc2 (isomer 8A1,2,3B1,3) + D-mannose
-
 ?

Organism

Organism UniProt Comment Textmining
Penicillium citrinum
-
-
-
Penicillium citrinum P31723
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and CM-Toyopearl 650M column chromatography Penicillium citrinum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Man9GlcNAc2 + H2O
-
 Penicillium citrinum Man8GlcNAc2 (isomer 8A1,2,3B1,3) + D-mannose
-
 ?

Subunits

Subunits Comment Organism
homodimer x-ray crystallography Penicillium citrinum

Synonyms

Synonyms Comment Organism
alpha1,2-mannosidase
-
 Penicillium citrinum
msdC
-
 Penicillium citrinum

General Information

General Information Comment Organism
physiological function comparison of the molecular structure of the Penicillium citrinum Golgi enzyme, and yeast and human endoplasmic reticulum enzymes. There is a greater degree of freedom to bind the oligosaccharide in the active site of the fungal enzyme than in the yeast and human ER alpha1,2-mannosidases Penicillium citrinum