Cloned (Comment) | Organism |
---|---|
enzyme-protein A-fusion protein is expressed in COS cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
deoxymannojirimycin | - |
Homo sapiens | |
EDTA | the enzyme inhibition by EDTA can be reversed by the addition of Ca2+ and to a lesser extent by Fe2+ and Mn2+ | Homo sapiens | |
EGTA | the enzyme inhibition by EGTA can be reversed by the addition of Ca2+ and to a lesser extent by Fe2+ and Mn2+ | Homo sapiens | |
kifunensine | - |
Homo sapiens | |
additional information | not inhibited by swainsonine | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Homo sapiens | 5783 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Man9GlcNAc2-Asn + H2O | Homo sapiens | - |
Man8GlcNAc2-Asn (isomer 8A1,2,3B1,3) + D-mannose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9UKM7 | - |
- |
Purification (Comment) | Organism |
---|---|
IgG-Sepharose bead chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Man9GlcNAc2-Asn + H2O | - |
Homo sapiens | Man8GlcNAc2-Asn (isomer 8A1,2,3B1,3) + D-mannose | - |
? | |
Man9GlcNAc2-pyridylamine + H2O | - |
Homo sapiens | Man8GlcNAc2-pyridylamine (isomer 8A1,2,3B1,3) + D-mannose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha1,2-mannosidase I | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | the enzyme is active between pH 6.0 and 8.0 | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes the first mannose trimming step in the processing of mammalian Asn-linked oligosaccharides | Homo sapiens |