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Literature summary for 3.2.1.113 extracted from

  • Gonzalez, D.; Karaveg, K.; Vandersall-Nairn, A.; Lal, A.; Moremen, K.
    Identification, expression, and characterization of a cDNA encoding human endoplasmic reticulum mannosidase I, the enzyme that catalyzes the first mannose trimming step in mammalian Asn-linked oligosaccharide biosynthesis (1999), J. Biol. Chem., 274, 21375-21386 .
    View publication on PubMed
Search for more literature for 3.2.1.113

Cloned(Commentary)

Cloned (Comment) Organism
enzyme-protein A-fusion protein is expressed in COS cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
deoxymannojirimycin
-
 Homo sapiens
EDTA the enzyme inhibition by EDTA can be reversed by the addition of Ca2+ and to a lesser extent by Fe2+ and Mn2+ Homo sapiens
EGTA the enzyme inhibition by EGTA can be reversed by the addition of Ca2+ and to a lesser extent by Fe2+ and Mn2+ Homo sapiens
kifunensine
-
 Homo sapiens
additional information not inhibited by swainsonine Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Homo sapiens 5783
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Man9GlcNAc2-Asn + H2O Homo sapiens
-
 Man8GlcNAc2-Asn (isomer 8A1,2,3B1,3) + D-mannose
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UKM7
-
-

Purification (Commentary)

Purification (Comment) Organism
IgG-Sepharose bead chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Man9GlcNAc2-Asn + H2O
-
 Homo sapiens Man8GlcNAc2-Asn (isomer 8A1,2,3B1,3) + D-mannose
-
 ?
Man9GlcNAc2-pyridylamine + H2O
-
 Homo sapiens Man8GlcNAc2-pyridylamine (isomer 8A1,2,3B1,3) + D-mannose
-
 ?

Synonyms

Synonyms Comment Organism
alpha1,2-mannosidase I
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6 8 the enzyme is active between pH 6.0 and 8.0 Homo sapiens

General Information

General Information Comment Organism
metabolism the enzyme catalyzes the first mannose trimming step in the processing of mammalian Asn-linked oligosaccharides Homo sapiens