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Literature summary for 3.2.1.113 extracted from
- Improved expression and characterization of recombinant human Golgi alpha1,2-mannosidase I isoforms (IA2 and IC) by Escherichia coli (2011), J. Biosci. Bioeng., 112, 14-19.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli JM109 (DE3) cells | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-deoxymannojirimycin | no activity at 0.01 mM; no activity at 0.01 mM | Homo sapiens |  |
| kifunensine | no activity at 0.01 mM; no activity at 0.01 mM | Homo sapiens | |
| additional information | isoform ManIA2 is not influenced by swainsonine | Homo sapiens | |
| swainsonine | isoform ManIC shows 86% activity at 0.5 mM swainsonine | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required for activity. Optimum concentration 1.5 mM or 15 mM. Isoform ManIC is stable in the absence of Ca2+, even though Ca2+ is also effective for activity | Homo sapiens | |
| Ca2+ | required for activity. Optimum concentration 15 mM. the activity of soform ManIA2 is significantly decreased after incubation at 30°C without Ca2+, which is about 30% compared with the activity of the enzyme after incubation at 5°C. The enzyme activity of ManIA2 increases with increasing Ca2+ concentration until 12 mM | Homo sapiens | |
| additional information | isoform ManIA2 shows no activity in the presence of Co2+, Mn2+, and Cu2+ | Homo sapiens | |
| additional information | isoform ManIC shows no activity in the presence of Co2+ and Mn2+ | Homo sapiens | |
| Zn2+ | the activity of isoform ManIC is 94% in the presence of 15 mM Zn2+ and 50% in the presence of 1.5 mM Zn2+ compared with that of the original solution | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
x * 58000, SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | O60476 | - |
- |
| Homo sapiens | Q9NR34 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Talon Co2+ affinity resin column chromatography | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Manalpha(1->2)Manalpha(1->2)Manalpha(1->3)[Manalpha(1->2)Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + H2O | - |
Homo sapiens | Manalpha(1->2)Manalpha(1->3)[Manalpha(1->2)Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + D-mannose | - |
? | |
| Manalpha(1->2)Manalpha(1->3)[Manalpha(1->2)Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + H2O | - |
Homo sapiens | Manalpha(1->3)[Manalpha(1->2)Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + D-mannose | - |
? | |
| Manalpha(1->3)[Manalpha(1->2)Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + H2O | - |
Homo sapiens | Manalpha(1->3)[Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + D-mannose | - |
? | |
| Manalpha(1->3)[Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + H2O | - |
Homo sapiens | Manalpha(1->3)[Manalpha(1->6)[Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + D-mannose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 58000, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Golgi alpha1,2-mannosidase I | - |
Homo sapiens |
| ManIA2 | isoform | Homo sapiens |
| ManIC | isoform | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
isoform ManIC | Homo sapiens |
| 40 | - |
isoform ManIA2 | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 30 | isoform ManIA2 shows more than 75% activity at 30°C in the presence of Ca2+, and more than 75% activity at 20°C in the absence of Ca2+ | Homo sapiens |
| 40 | - |
isoform ManIC shows more than 75% activity at 40°C in the absence and presence of Ca2+ | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.5 | isoform ManIC | Homo sapiens |
| 6.5 | 7 | isoform ManIA2 | Homo sapiens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6 | isoform ManIC is stable (more than 75% activity) in the pH range of 5.5-6.0 at 37°C for 90 min | Homo sapiens |
| 6.5 | 8 | isoform ManIA2 is stable (more than 75% activity) in the pH range of 6.5-8.0 at 37°C for 90 min | Homo sapiens |
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