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Literature summary for 3.2.1.113 extracted from

  • Kajiura, H.; Koiwa, H.; Nakazawa, Y.; Okazawa, A.; Kobayashi, A.; Seki, T.; Fujiyama, K.
    Two Arabidopsis thaliana Golgi alpha-mannosidase I enzymes are responsible for plant N-glycan maturation (2010), Glycobiology, 20, 235-247.
    View publication on PubMed
Search for more literature for 3.2.1.113

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf9 cells as insoluble forms and in Escherichia coli as soluble forms Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
1-deoxymannojirimycin isozyme MANIa shows 20% residual activity at 0.1 mM 1-deoxymannojirimycin, isozyme MANIb shows 45% residual activity at 0.1 mM 1-deoxymannojirimycin Arabidopsis thaliana
EDTA isozyme MANIa shows 20% residual activity at 1 mM EDTA, isozyme MANIb shows 35% residual activity at 1 mM EDTA, isozyme MANIb activity in the presence of 1 mM EDTA is restored by the addition of Ca2+ Arabidopsis thaliana
additional information not inhibited by swainsonine Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus
-
 Arabidopsis thaliana 5794
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required for activity, isozyme MANIa shows 450% relative activity at 15 mM Ca2+, isozyme MANIb shows 600% relative activity at 15 mM Ca2+ Arabidopsis thaliana
additional information Co2+, Cu2+, Mg2+, Mn2+, and Zn2+ have no effect on activity Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 His-tagged isozymes MANIa and MANIb, SDS-PAGE Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Co2+-affinity column chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Man8GlcNAc2 + H2O
-
 Arabidopsis thaliana Man5GlcNAc2 + D-mannose
-
 ?
Man9GlcNAc2 + 4 H2O
-
 Arabidopsis thaliana Man5GlcNAc2 + 4 D-mannose
-
 ?
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)]Manalpha(1-6)]Manbeta(1-4)GlcNACbeta(1-4)GlcNAc + H2O
-
 Arabidopsis thaliana Manalpha(1-3)[Manalpha(1-3)[Manalpha(1-6)]Manalpha(1-6)]Manbeta(1-4)GlcNACbeta(1-4)GlcNAc + 4 D-mannose
-
 ?
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
 Arabidopsis thaliana Manalpha(1-3)[Manalpha(1-3)[Manalpha(1-6)]Manalpha(1-6)]Manbeta(1-4)GlcNACbeta(1-4)GlcNAc + 3 D-mannose
-
 ?

Synonyms

Synonyms Comment Organism
Golgi alpha-mannosidase I
-
 Arabidopsis thaliana
ManI
-
 Arabidopsis thaliana
MANIa isozyme Arabidopsis thaliana
MANIb isozyme Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 isozymes MANIa and MANIb Arabidopsis thaliana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 isozymes MANIa and MANIb are relatively stable at lower temperature and still retain 50% of their original activities after incubation at 25°C for 1 h and show a significant decrease of activities at higher temperatures with 10% activity at 30°C Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 isozyme MANIb Arabidopsis thaliana
7
-
 isozyme MANIa Arabidopsis thaliana

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 6.5 isozyme MANIb Arabidopsis thaliana
5 10 isozyme MANIa Arabidopsis thaliana

General Information

General Information Comment Organism
physiological function either isozyme MANIa or MANIb can function as the Golgi alpha-mannosidase I that produces the Man5GlcNAc2 N-glycan structure necessary for complex N-glycan synthesis Arabidopsis thaliana