Literature summary for 3.2.1.113 extracted from

Lunetta, J.M.; Simmons, K.A.; Johnson, S.M.; Pappagianis, D.
Molecular cloning and expression of a cDNA encoding a Coccidioides posadasii 1,2-alpha-mannosidase identified in the coccidioidal T27K vaccine by immunoproteomic methods (2007), Ann. N. Y. Acad. Sci., 1111, 164-180.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in TOP10 chemically competent Escherichia coli cells	Coccidioides posadasii

Inhibitors

Inhibitors	Comment	Organism	Structure
deoxymannojirimycin hydrochloride	inhibitor at a final concentration of 0.5 mM, reduces the activity by 80%	Coccidioides posadasii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
56920	-	theoretical molecular weight. SDS-PAGE, Western blot analysis. A highly purified fraction is obtained after FPLC	Coccidioides posadasii

Organism

Organism	UniProt	Comment	Textmining
Coccidioides posadasii	Q3HYC1	very similar (> 60%) to other fungal 1,2-alpha-mannosidases; silveira strain, formerly known as the non-California Coccidioides immitis	-

Purification (Commentary)

Purification (Comment)	Organism
protein identification by tandem mass spectrometry. Gel filtration	Coccidioides posadasii

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	detected in the coccidioidal T27K vaccine	Coccidioides posadasii	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
methyl 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside + H2O	used in a spectrophotometric assay	Coccidioides posadasii	? + D-mannose	-	?

Synonyms

Synonyms	Comment	Organism
1,2-alpha-mannosidase	-	Coccidioides posadasii

pI Value

Organism	Comment	pI Value Maximum	pI Value
Coccidioides posadasii	-	-	4.84

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Release 2023.1 (January 2023)