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Literature summary for 3.2.1.113 extracted from

  • Kobata, A.; Amano, J.
    alpha-Mannosidases I and II from Aspergillus saitoi (1987), Methods Enzymol., 138, 779-785.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.25
-
Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
-
Aspergillus phoenicis

Metals/Ions

Metals/Ions Comment Organism Structure
divalent cations not Aspergillus phoenicis

Organism

Organism UniProt Comment Textmining
Aspergillus phoenicis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus phoenicis

Source Tissue

Source Tissue Comment Organism Textmining
culture medium commercially available under the name of Morushin Aspergillus phoenicis
-
mycelium commercially available under the name of Morushin Aspergillus phoenicis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
34.6
-
37°C, pH 5.0 Aspergillus phoenicis

Storage Stability

Storage Stability Organism
-20°C, in 10 mM sodium acetate buffer, pH 5, with or without 1 mM CaCl2, at least 4 months Aspergillus phoenicis
4°C, in 10 mM sodium acetate buffer, pH 5, with or without 1 mM CaCl2, at least 4 months Aspergillus phoenicis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(Manalpha(1-2))nManalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3)) Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
Aspergillus phoenicis Manalpha1-6(Manalpha1-3)Manalpha1-6(Manalpha1-3)Manbeta1-4GlcNAc beta1-4GlcNAc + D-mannose
-
?
Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O
-
Aspergillus phoenicis ? + D-mannose
-
?
additional information no hydrolysis of alpha1,3-linkages Aspergillus phoenicis ?
-
?
additional information no hydrolysis of alpha1,6-linkages Aspergillus phoenicis ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Aspergillus phoenicis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Aspergillus phoenicis