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Literature summary for 3.2.1.111 extracted from

  • Saumonneau, A.; Champion, E.; Peltier-Pain, P.; Molnar-Gabor, D.; Hendrickx, J.; Tran, V.; Hederos, M.; Dekany, G.; Tellier, C.
    Design of an alpha-L-transfucosidase for the synthesis of fucosylated HMOs (2016), Glycobiology, 26, 261-269 .
    View publication on PubMed
Search for more literature for 3.2.1.111

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia XL1 blue cells Bifidobacterium longum subsp. infantis

Protein Variants

Protein Variants Comment Organism
F34H/L321P the mutant exhibits increased transfucosylation activity compared to the wild type enzyme Bifidobacterium longum subsp. infantis
F34I/L321P the mutant exhibits increased transfucosylation activity compared to the wild type enzyme Bifidobacterium longum subsp. infantis
F34Y/L321P the mutant exhibits increased transfucosylation activity compared to the wild type enzyme Bifidobacterium longum subsp. infantis
L321P the mutant exhibits increased transfucosylation activity compared to the wild type enzyme Bifidobacterium longum subsp. infantis

Organism

Organism UniProt Comment Textmining
Bifidobacterium longum subsp. infantis B7GNN8
-
-
Bifidobacterium longum subsp. infantis ATCC 15697 B7GNN8
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Bifidobacterium longum subsp. infantis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-O-fucosyllactose + 2'-fucosyllactose
-
 Bifidobacterium longum subsp. infantis lactodifucotetraose + ?
-
 ?
3-O-fucosyllactose + 2'-fucosyllactose
-
 Bifidobacterium longum subsp. infantis ATCC 15697 lactodifucotetraose + ?
-
 ?
3-O-fucosyllactose + lacto-N-fucopentaose I
-
 Bifidobacterium longum subsp. infantis lacto-N-difucohexaose + lactose
-
 ?
3-O-fucosyllactose + lacto-N-fucopentaose I
-
 Bifidobacterium longum subsp. infantis ATCC 15697 lacto-N-difucohexaose + lactose
-
 ?
3-O-fucosyllactose + lacto-N-neotetraose
-
 Bifidobacterium longum subsp. infantis lacto-N-fucopentaose III + lactose
-
 ?
3-O-fucosyllactose + lacto-N-neotetraose
-
 Bifidobacterium longum subsp. infantis ATCC 15697 lacto-N-fucopentaose III + lactose
-
 ?
3-O-fucosyllactose + lacto-N-tetraose
-
 Bifidobacterium longum subsp. infantis lacto-N-fucopentaose-II + lactose
-
 ?
3-O-fucosyllactose + lacto-N-tetraose
-
 Bifidobacterium longum subsp. infantis ATCC 15697 lacto-N-fucopentaose-II + lactose
-
 ?

Synonyms

Synonyms Comment Organism
AfcB
-
 Bifidobacterium longum subsp. infantis
alpha(1,3/4)-transfucosidase
-
 Bifidobacterium longum subsp. infantis
alphafuc
-
 Bifidobacterium longum subsp. infantis