Any feedback?
Literature summary for 3.2.1.111 extracted from
- Purification of an almond emulsin fucosidase on Cibacron blue-Sepharose and demonstration of its activity toward fucose-containing glycoproteins (1982), J. Biol. Chem., 257, 8205-8210.
General Stability
| General Stability | Organism |
|---|---|
| decreasing ionic strength inactivates | Prunus dulcis |
| freeze-thawing results in 30% loss of initial activity | Prunus dulcis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | strong | Prunus dulcis |  |
| L-fucose | - |
Prunus dulcis | |
| malonate | above 20 mM | Prunus dulcis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | slight activation | Prunus dulcis | |
| Mg2+ | slight activation | Prunus dulcis | |
| additional information | no activation by Mn2+ | Prunus dulcis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 73000 | - |
gel filtration | Prunus dulcis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Prunus dulcis | - |
almond | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity chromatography on Blue Sepharose | Prunus dulcis |
| partial | Prunus dulcis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| emulsin | commercially available | Prunus dulcis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0645 | - |
- |
Prunus dulcis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, less than 10% loss of activity within 6 months | Prunus dulcis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| asialoorosomucoid + H2O | specifically hydrolyzes fucose in alpha-1,3-linkage to N-acetylglucosamine of Asn-linked oligosaccharide chain branches | Prunus dulcis | ? | - |
? | |
| lactoferrin + H2O | hydrolyzes only alpha-1,3-linkages between fucose and N-acetylglucosamine | Prunus dulcis | ? | - |
? | |
| additional information | no substrates are alpha2-macroglobulin (MW 72600), bovine IgG glycopeptides or milk oligosaccharide 2'-fucosyllactose | Prunus dulcis | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Prunus dulcis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.3 | - |
- |
Prunus dulcis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.3 | 5.8 | about half-maximal activity at pH 4.3 and about 80% of maximal activity at pH 5.8 | Prunus dulcis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
