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Literature summary for 3.2.1.11 extracted from

  • Netsopa, S.; Niamsanit, S.; Araki, T.; Kongkeitkajorn, M.; Milintawisamai, N.
    Purification and characterization including dextran hydrolysis of dextranase from Aspergillus allahabadii X26 (2019), Sugar Tech., 21, 329-340 .
No PubMed abstract available
Search for more literature for 3.2.1.11

Activating Compound

Activating Compound Comment Organism Structure
Urea 104.09% activity at 1 mM Aspergillus allahabadii

Application

Application Comment Organism
food industry the enzyme is used for dextran elimination in sugar production process Aspergillus allahabadii

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 92.2% residual activity at 10 mM Aspergillus allahabadii
Cu2+ 78.62% residual activity at 10 mM Aspergillus allahabadii
EDTA 91.76% residual activity at 10 mM Aspergillus allahabadii
Fe2+ 98.71% residual activity at 10 mM Aspergillus allahabadii
Mg2+ 94.4% residual activity at 10 mM Aspergillus allahabadii
SDS 91.08% residual activity at 10 mM Aspergillus allahabadii
Tris 91.29% residual activity at 10 mM Aspergillus allahabadii
Zn2+ 94.26% residual activity at 10 mM Aspergillus allahabadii

Metals/Ions

Metals/Ions Comment Organism Structure
Al3+ 102.62% activity at 1 mM Aspergillus allahabadii
Ca2+ 116.54% activity at 5 mM Aspergillus allahabadii
Co2+ 129.42% activity at 5 mM Aspergillus allahabadii
Fe2+ 104.53% activity at 1 mM Aspergillus allahabadii
Mn2+ 167.32% activity at 1 mM Aspergillus allahabadii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dextran + H2O Aspergillus allahabadii the enzyme only has specificity to the alpha(1->6) glycosidic bond of dextran isomaltooligosaccharides
-
 ?
dextran + H2O Aspergillus allahabadii X26 the enzyme only has specificity to the alpha(1->6) glycosidic bond of dextran isomaltooligosaccharides
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus allahabadii
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-
-
Aspergillus allahabadii X26
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Aspergillus allahabadii

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and Sephacryl S-300 HR gel filtration Aspergillus allahabadii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dextran + H2O the enzyme only has specificity to the alpha(1->6) glycosidic bond of dextran Aspergillus allahabadii isomaltooligosaccharides
-
 ?
dextran + H2O the enzyme only has specificity to the alpha(1->6) glycosidic bond of dextran Aspergillus allahabadii X26 isomaltooligosaccharides
-
 ?
dextran T10 + H2O 88.64% activity compared to dextran T250 Aspergillus allahabadii isomaltose + isomaltotriose + isomaltooligosaccharides
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 ?
dextran T10 + H2O 88.64% activity compared to dextran T250 Aspergillus allahabadii X26 isomaltose + isomaltotriose + isomaltooligosaccharides
-
 ?
dextran T2000 + H2O 87.48% activity compared to dextran T250 Aspergillus allahabadii isomaltose + isomaltotriose + isomaltooligosaccharides
-
 ?
dextran T2000 + H2O 87.48% activity compared to dextran T250 Aspergillus allahabadii X26 isomaltose + isomaltotriose + isomaltooligosaccharides
-
 ?
dextran T250 + H2O 100% activity Aspergillus allahabadii isomaltose + isomaltotriose + isomaltooligosaccharides
-
 ?
dextran T250 + H2O 100% activity Aspergillus allahabadii X26 isomaltose + isomaltotriose + isomaltooligosaccharides
-
 ?
dextran T500 + H2O 90.78% activity compared to dextran T250 Aspergillus allahabadii isomaltose + isomaltotriose + isomaltooligosaccharides
-
 ?
dextran T60-90 + H2O 90.19% activity compared to dextran T250 Aspergillus allahabadii isomaltose + isomaltotriose + isomaltooligosaccharides
-
 ?
additional information no activity with starch, cellulose, glycol chitin, chitin, Sephadex G10, sucrose, and maltose Aspergillus allahabadii ?
-
 ?
additional information no activity with starch, cellulose, glycol chitin, chitin, Sephadex G10, sucrose, and maltose Aspergillus allahabadii X26 ?
-
 ?
Sephacryl S-300 + H2O 4.02% activity compared to dextran T250 Aspergillus allahabadii ?
-
 ?

Subunits

Subunits Comment Organism
? x * 66000, SDS-PAGE Aspergillus allahabadii

Synonyms

Synonyms Comment Organism
alpha-1,6-D-glucan-6-glucanohydrolase
-
 Aspergillus allahabadii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Aspergillus allahabadii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 65 the enzyme activity successively increases with temperature from 25 to 55°C. Rising the temperatures from 55 to 60 and 65°C results in a decrease in enzyme activity by 25% and 66%, respectively Aspergillus allahabadii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 75 the enzyme exhibits thermostability in the range of 20-50°C over an incubation period of 1.5 h. The enzyme loses more than 50% activity at 55°C and 100% activity over the range of 60-75°C. The half-life at 55°C is 52 min Aspergillus allahabadii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
-
 Aspergillus allahabadii

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9 the enzyme exhibits stability with more than 80% relative activity at pH 5.0-9.0 Aspergillus allahabadii