Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.11 extracted from

  • Ren, W.; Liu, L.; Gu, L.; Yan, W.; Feng, Y.L.; Dong, D.; Wang, S.; Lyu, M.; Wang, C.
    Crystal structure of GH49 dextranase from Arthrobacter oxidans KQ11 identification of catalytic base and improvement of thermostability using semirational design based on B-factors (2019), J. Agric. Food Chem., 67, 4355-4366 .
    View publication on PubMed
Search for more literature for 3.2.1.11

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudarthrobacter oxydans

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.2 M MgCl2, 0.1 M HEPES (pH 7.5), and 30% (w/v) polyethylene glycol 400 Pseudarthrobacter oxydans

Protein Variants

Protein Variants Comment Organism
A356F the mutant exhibits improved thermostability compared to the wild type enzyme Pseudarthrobacter oxydans
G355F the mutant exhibits improved thermostability compared to the wild type enzyme Pseudarthrobacter oxydans
S354F the mutant exhibits improved thermostability compared to the wild type enzyme Pseudarthrobacter oxydans
S357F the mutant exhibits improved thermostability compared to the wild type enzyme Pseudarthrobacter oxydans
S357F/G355F the mutant exhibits improved thermostability compared to the wild type enzyme Pseudarthrobacter oxydans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dextran + H2O Pseudarthrobacter oxydans
-
 D-glucose + isomaltose + isomaltooligosaccharides
-
 ?
dextran + H2O Pseudarthrobacter oxydans KQ11
-
 D-glucose + isomaltose + isomaltooligosaccharides
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudarthrobacter oxydans A0A059VLI3
-
-
Pseudarthrobacter oxydans KQ11 A0A059VLI3
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and ion-exchange column chromatography Pseudarthrobacter oxydans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dextran + H2O
-
 Pseudarthrobacter oxydans D-glucose + isomaltose + isomaltooligosaccharides
-
 ?
dextran + H2O
-
 Pseudarthrobacter oxydans KQ11 D-glucose + isomaltose + isomaltooligosaccharides
-
 ?
dextran T2000 + H2O
-
 Pseudarthrobacter oxydans isomaltose + isomaltotriose + isomaltooligosaccharides
-
 ?
dextran T2000 + H2O
-
 Pseudarthrobacter oxydans KQ11 isomaltose + isomaltotriose + isomaltooligosaccharides
-
 ?

Subunits

Subunits Comment Organism
? x * 66200, SDS-PAGE Pseudarthrobacter oxydans

Synonyms

Synonyms Comment Organism
alpha-1,6-D-glucan 6-glucanohydrolase
-
 Pseudarthrobacter oxydans
Dex
-
 Pseudarthrobacter oxydans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Pseudarthrobacter oxydans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 60 the enzyme has a thermostable temperature of 30°C, a half-life of 30 min at 55°C and shows 9.66% residual activity after 30 min at 60°C Pseudarthrobacter oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Pseudarthrobacter oxydans

pH Range

pH Minimum pH Maximum Comment Organism
4.5 9.5 the enzyme is inactive below pH 4.5 and above pH 9.5 Pseudarthrobacter oxydans

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 8.5 the enzyme has a stable pH range from 5.5 to 8.5 Pseudarthrobacter oxydans