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Literature summary for 3.2.1.11 extracted from
- Purification, characterization and degradation performance of a novel dextranase from Penicillium cyclopium CICC-4022 (2019), Int. J. Mol. Sci., 20, 1360 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| SDS | 106.16% activity at 1 mM | Penicillium cyclopium |  |
| Tris | 103.79% activity at 1 mM | Penicillium cyclopium | |
| Urea | 112.79% activity at 1 mM | Penicillium cyclopium | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 40.73% residual activity at 10 mM | Penicillium cyclopium | |
| Cr2+ | 48.25% residual activity at 10 mM | Penicillium cyclopium | |
| Cu2+ | 16.41% residual activity at 10 mM | Penicillium cyclopium | |
| EDTA | 80.58% residual activity at 5 mM | Penicillium cyclopium | |
| Fe3+ | 64.61% residual activity at 5 mM | Penicillium cyclopium | |
| Ni2+ | 67.71% residual activity at 10 mM | Penicillium cyclopium | |
| Pb2+ | 20.39% residual activity at 10 mM | Penicillium cyclopium | |
| SDS | 34.15% residual activity at 10 mM | Penicillium cyclopium | |
| Zn2+ | 16.85% residual activity at 10 mM | Penicillium cyclopium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.45 | - |
dextran T2000 | at pH 5.0 and 50°C | Penicillium cyclopium | |
| 2.61 | - |
dextran T70 | at pH 5.0 and 50°C | Penicillium cyclopium | |
| 2.72 | - |
dextran T5 | at pH 5.0 and 50°C | Penicillium cyclopium |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | 109.95% activity at 10 mM | Penicillium cyclopium | |
| Li+ | 116.28% activity at 1 mM | Penicillium cyclopium | |
| Na+ | 111.48% activity at 5 mM | Penicillium cyclopium | |
| NH4+ | 107.58% activity at 1 mM | Penicillium cyclopium | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dextran + H2O | Penicillium cyclopium | the enzyme specially catalyzes the endohydrolysis of alpha-(1,6)-D-glycoside linkages at random sites of dextran | isomaltose + isomaltotriose + D-glucose + low molecular-mass polysaccharides | - |
? | |
| dextran + H2O | Penicillium cyclopium CICC-4022 | the enzyme specially catalyzes the endohydrolysis of alpha-(1,6)-D-glycoside linkages at random sites of dextran | isomaltose + isomaltotriose + D-glucose + low molecular-mass polysaccharides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium cyclopium | - |
- |
- |
| Penicillium cyclopium CICC-4022 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation and Sepharose column chromatography | Penicillium cyclopium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dextran + H2O | the enzyme specially catalyzes the endohydrolysis of alpha-(1,6)-D-glycoside linkages at random sites of dextran | Penicillium cyclopium | isomaltose + isomaltotriose + D-glucose + low molecular-mass polysaccharides | - |
? | |
| dextran + H2O | the enzyme specially catalyzes the endohydrolysis of alpha-(1,6)-D-glycoside linkages at random sites of dextran | Penicillium cyclopium CICC-4022 | isomaltose + isomaltotriose + D-glucose + low molecular-mass polysaccharides | - |
? | |
| dextran T20 + H2O | 90.64% activity compared to dextran T70 | Penicillium cyclopium | isomaltose + isomaltotriose + D-glucose | - |
? | |
| dextran T20 + H2O | 90.64% activity compared to dextran T70 | Penicillium cyclopium CICC-4022 | isomaltose + isomaltotriose + D-glucose | - |
? | |
| dextran T2000 + H2O | 97% activity compared to dextran T70 | Penicillium cyclopium | isomaltose + isomaltotriose + D-glucose | - |
? | |
| dextran T2000 + H2O | 97% activity compared to dextran T70 | Penicillium cyclopium CICC-4022 | isomaltose + isomaltotriose + D-glucose | - |
? | |
| dextran T3 + H2O | 65.17% activity compared to dextran T70 | Penicillium cyclopium | isomaltose + isomaltotriose + D-glucose | - |
? | |
| dextran T40 + H2O | 98.5% activity compared to dextran T70 | Penicillium cyclopium | isomaltose + isomaltotriose + D-glucose | - |
? | |
| dextran T5 + H2O | 65.54% activity compared to dextran T70 | Penicillium cyclopium | isomaltose + isomaltotriose + D-glucose | - |
? | |
| dextran T500 + H2O | 99.62% activity compared to dextran T70 | Penicillium cyclopium | isomaltose + isomaltotriose + D-glucose | - |
? | |
| dextran T70 + H2O | 100% activity | Penicillium cyclopium | isomaltose + isomaltotriose + D-glucose | - |
? | |
| additional information | the enzyme has no effects on cellulose, sucrose, chitin, chitosan and beta-cyclodextrin | Penicillium cyclopium | ? | - |
? | |
| additional information | the enzyme has no effects on cellulose, sucrose, chitin, chitosan and beta-cyclodextrin | Penicillium cyclopium CICC-4022 | ? | - |
? | |
| soluble starch + H2O | 20.83% activity compared to dextran T70 | Penicillium cyclopium | ? | - |
? | |
| soluble starch + H2O | 20.83% activity compared to dextran T70 | Penicillium cyclopium CICC-4022 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 66000, SDS-PAGE | Penicillium cyclopium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 1,6-alpha-D-glucan-6-glucanohydrolase | - |
Penicillium cyclopium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Penicillium cyclopium |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 60 | when temperature varying from 35 to 60°C, the relative activity is always higher than 54.55% | Penicillium cyclopium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 50 | after 60 min 45°C and 50°C, the dextranase activity remains stable with the values being 98.09% and 62.21% of relative enzymatic activity, respectively | Penicillium cyclopium |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 208.45 | - |
dextran T5 | at pH 5.0 and 50°C | Penicillium cyclopium | |
| 211.97 | - |
dextran T70 | at pH 5.0 and 50°C | Penicillium cyclopium | |
| 223.53 | - |
dextran T2000 | at pH 5.0 and 50°C | Penicillium cyclopium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Penicillium cyclopium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6 | with pH ranged from 3.0 to 6.0, the relative enzyme activity is higher than 72.67% | Penicillium cyclopium |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | 7 | the relative enzymatic activity of dextranase stored at pH 2.5-7.0 for 1 h and 24 h, is 89.22% and 84.58%, respectively | Penicillium cyclopium |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 76.636 | - |
dextran T5 | at pH 5.0 and 50°C | Penicillium cyclopium | |
| 81.215 | - |
dextran T70 | at pH 5.0 and 50°C | Penicillium cyclopium | |
| 91.237 | - |
dextran T2000 | at pH 5.0 and 50°C | Penicillium cyclopium |
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