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Literature summary for 3.2.1.11 extracted from

  • Yang, L.; Zhou, N.; Tian, Y.
    Purification, characterization, and biocatalytic potential of a novel dextranase from Chaetomium globosum (2018), Biotechnol. Lett., 40, 1407-1418 .
    View publication on PubMed
Search for more literature for 3.2.1.11

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 89.21% residual activity at 10 mM Chaetomium globosum
Co2+ 72.51% residual activity at 10 mM Chaetomium globosum
Cu2+ 28.55% residual activity at 10 mM Chaetomium globosum
EDTA 89.55% residual activity at 5 mM Chaetomium globosum
Fe3+ 87.31% residual activity at 10 mM Chaetomium globosum
K+ 80.2% residual activity at 1 mM Chaetomium globosum
Mn2+ 85.61% residual activity at 1 mM Chaetomium globosum
additional information Zn2+, Co2+, Ni2+, Fe3+ and EDTA show no obvious inhibition at 1 mM Chaetomium globosum
Ni2+ 88.82% residual activity at 10 mM Chaetomium globosum
SDS 8.61% residual activity at 5 mM Chaetomium globosum
Tris 86.53% residual activity at 10 mM Chaetomium globosum
Zn2+ 76.28% residual activity at 10 mM Chaetomium globosum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
168.27
-
 dextran T20 at pH 5.5 and 50°C Chaetomium globosum
172.22
-
 dextran T40 at pH 5.5 and 50°C Chaetomium globosum
327.19
-
 dextran T2000 at pH 5.5 and 50°C Chaetomium globosum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 10 mM Mg2+ enhances dextranase activity by 118.73% Chaetomium globosum
Mn2+ 10 mM Mn2+ enhances dextranase activity by 134.44% Chaetomium globosum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dextran + H2O Chaetomium globosum
-
 isomaltooligosaccharides
-
 ?

Organism

Organism UniProt Comment Textmining
Chaetomium globosum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and Sephadex G75 gel filtration Chaetomium globosum

Storage Stability

Storage Stability Organism
4°C to -20°C, 0.02 M acetate buffer (pH 5.5), over 2 months, no loss of activity Chaetomium globosum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dextran + H2O
-
 Chaetomium globosum isomaltooligosaccharides
-
 ?
dextran T20 + H2O 68.64% activity compared to dextran T2000 Chaetomium globosum isomaltooligosaccharides
-
 ?
dextran T2000 + H2O very high hydrolytic affinity, 100% activity with 3% (w/v) dextran T2000 Chaetomium globosum isomaltooligosaccharides
-
 ?
dextran T40 + H2O 72.96% activity compared to dextran T2000 Chaetomium globosum isomaltooligosaccharides
-
 ?
additional information no activity toward Sepharose 6B, beta-cyclodextrin, chitosan, maltose, or sucrose Chaetomium globosum ?
-
 ?

Subunits

Subunits Comment Organism
? x * 53000, SDS-PAGE Chaetomium globosum

Synonyms

Synonyms Comment Organism
alpha-1,6-D-glucan-6-glucanohydrolase
-
 Chaetomium globosum
Alpha-1,6-glucan-6-glucanohydrolase
-
 Chaetomium globosum
alpha-glucanase
-
 Chaetomium globosum
endodextranase
-
 Chaetomium globosum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Chaetomium globosum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 50 the enzyme retains greater than 85% residual activity after storage at 20-50°C (pH 5.5) for 1 h. The enzyme is thermally stable at temperatures below 50°C and has a half-life of 45 days at 28°C Chaetomium globosum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Chaetomium globosum

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 7 the enzyme activity is extremely stable at pH 4.5-7.0 Chaetomium globosum