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Literature summary for 3.2.1.11 extracted from

  • Klahan, P.; Okuyama, M.; Jinnai, K.; Ma, M.; Kikuchi, A.; Kumagai, Y.; Tagami, T.; Kimura, A.
    Engineered dextranase from Streptococcus mutans enhances the production of longer isomaltooligosaccharides (2018), Biosci. Biotechnol. Biochem., 82, 1480-1487 .
    View publication on PubMed
Search for more literature for 3.2.1.11

Protein Variants

Protein Variants Comment Organism
T558H the mutation affects the hydrolytic activities of the enzyme. The mutant increases the proportion of isomaltooligosaccharide with degrees of polymerization of 5 in hydrolysates following reactions with 4 mg/ml dextran Streptococcus mutans
T563N the mutation affects the hydrolytic activities of the enzyme Streptococcus mutans
W279A the mutation affects the hydrolytic activities of the enzyme Streptococcus mutans
W279A/T563N the mutations affect the hydrolytic activities of the enzyme Streptococcus mutans
W279F the mutation affects the hydrolytic activities of the enzyme Streptococcus mutans
W279F/T563N the mutations affect the hydrolytic activities of the enzyme Streptococcus mutans

Organism

Organism UniProt Comment Textmining
Streptococcus mutans Q54443
-
-
Streptococcus mutans ATCC 700610 Q54443
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.7
-
 mutant enzyme W279F/T563N, at pH 5.0 and 35°C Streptococcus mutans
6.1
-
 mutant enzyme W279A/T563N, at pH 5.0 and 35°C Streptococcus mutans
11
-
 mutant enzyme T558H, at pH 5.0 and 35°C Streptococcus mutans
27
-
 mutant enzyme T563N, at pH 5.0 and 35°C Streptococcus mutans
43
-
 mutant enzyme W279A, at pH 5.0 and 35°C Streptococcus mutans
46
-
 mutant enzyme W279F, at pH 5.0 and 35°C Streptococcus mutans
58
-
 wild type enzyme, at pH 5.0 and 35°C Streptococcus mutans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-isomaltohexoside + H2O
-
 Streptococcus mutans ?
-
 ?
4-nitrophenyl alpha-isomaltohexoside + H2O
-
 Streptococcus mutans ATCC 700610 ?
-
 ?
4-nitrophenyl alpha-isomaltopentoside + H2O
-
 Streptococcus mutans ?
-
 ?
4-nitrophenyl alpha-isomaltopentoside + H2O
-
 Streptococcus mutans ATCC 700610 ?
-
 ?
4-nitrophenyl alpha-isomaltotetraoside + H2O
-
 Streptococcus mutans ?
-
 ?
4-nitrophenyl alpha-isomaltotetraoside + H2O
-
 Streptococcus mutans ATCC 700610 ?
-
 ?
dextran + H2O with 4 mg/ml dextran, isomaltooligosaccharides with degrees of polymerization of 3 and 4 are present at the beginning of the reaction, and D-glucose and isomaltose are produced by the end of the reaction Streptococcus mutans isomaltose + isomaltotriose + D-glucose + isomaltooligosaccharides
-
 ?
dextran + H2O with 4 mg/ml dextran, isomaltooligosaccharides with degrees of polymerization of 3 and 4 are present at the beginning of the reaction, and D-glucose and isomaltose are produced by the end of the reaction Streptococcus mutans ATCC 700610 isomaltose + isomaltotriose + D-glucose + isomaltooligosaccharides
-
 ?
isomaltohexaose + H2O
-
 Streptococcus mutans D-glucose + isomaltose + isomaltotriose + isomaltotetraose + isomaltopentaose
-
 ?
additional information no activity toward 4-nitrophenyl alpha-isomaltotrioside Streptococcus mutans ?
-
 ?
additional information no activity toward 4-nitrophenyl alpha-isomaltotrioside Streptococcus mutans ATCC 700610 ?
-
 ?

Synonyms

Synonyms Comment Organism
6-alpha-D-glucan 6-glucanohydrolase
-
 Streptococcus mutans
Dex
-
 Streptococcus mutans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 the enzyme is stable at temperatures below 40°C Streptococcus mutans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
29
-
 Dextran mutant enzyme T558H, at pH 5.0 and 35°C Streptococcus mutans
50
-
 Dextran mutant enzyme W279F/T563N, at pH 5.0 and 35°C Streptococcus mutans
57
-
 Dextran mutant enzyme T563N, at pH 5.0 and 35°C Streptococcus mutans
77
-
 Dextran wild type enzyme, at pH 5.0 and 35°C Streptococcus mutans
98
-
 Dextran mutant enzyme W279A/T563N, at pH 5.0 and 35°C Streptococcus mutans
99
-
 Dextran mutant enzyme W279A, at pH 5.0 and 35°C Streptococcus mutans
110
-
 Dextran mutant enzyme W279F, at pH 5.0 and 35°C Streptococcus mutans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Streptococcus mutans

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 9 the enzyme is stable between pH 4.0 and 9.0 Streptococcus mutans