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Literature summary for 3.2.1.11 extracted from

  • Zhang, Z.; Liu, J.; Ma, S.; Lu, H.; Hang, F.; Huang, P.; Li, K.
    Enhancement of catalytic performance of alpha-dextranase from Chaetomium gracile through optimization and suitable shear force (2018), Sugar Tech., 20, 78-87 .
No PubMed abstract available

Application

Application Comment Organism
food industry alpha-dextranase removes dextran by 46.6% in mixed sugarcane juice and 14.1% in clarified sugarcane juice Achaetomiella gracilis
synthesis alpha-dextranase activity reaches 242.8 U/ml when fermentation conditions are 29°C, pH 6.0 and 220 rpm. Addition of glass beads to fermentation medium after 12 h improves enzyme activity by 135.5% Achaetomiella gracilis

Inhibitors

Inhibitors Comment Organism Structure
SDS 5 mM, 2 h, 2% residual activity Achaetomiella gracilis
Triton X-100 5 mM, 2 h, 80% residual activity Achaetomiella gracilis
Tween-20 5 mM, 2 h, 90% residual activity Achaetomiella gracilis
Tween-80 5 mM, 2 h, 67% residual activity Achaetomiella gracilis

Organism

Organism UniProt Comment Textmining
Achaetomiella gracilis
-
-
-
Achaetomiella gracilis CGMCC3 3783
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Achaetomiella gracilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12569
-
pH 6.0, 55°C Achaetomiella gracilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dextran T20 + H2O
-
Achaetomiella gracilis ?
-
?
dextran T20 + H2O
-
Achaetomiella gracilis CGMCC3 3783 ?
-
?
dextran T2000 + H2O
-
Achaetomiella gracilis ?
-
?
dextran T2000 + H2O
-
Achaetomiella gracilis CGMCC3 3783 ?
-
?
dextran T500 + H2O
-
Achaetomiella gracilis ?
-
?
dextran T500 + H2O
-
Achaetomiella gracilis CGMCC3 3783 ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Achaetomiella gracilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70
-
rapid loss of activity above Achaetomiella gracilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
2 h, 90% residual activity Achaetomiella gracilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Achaetomiella gracilis