Application | Comment | Organism |
---|---|---|
food industry | alpha-dextranase removes dextran by 46.6% in mixed sugarcane juice and 14.1% in clarified sugarcane juice | Achaetomiella gracilis |
synthesis | alpha-dextranase activity reaches 242.8 U/ml when fermentation conditions are 29°C, pH 6.0 and 220 rpm. Addition of glass beads to fermentation medium after 12 h improves enzyme activity by 135.5% | Achaetomiella gracilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
SDS | 5 mM, 2 h, 2% residual activity | Achaetomiella gracilis | |
Triton X-100 | 5 mM, 2 h, 80% residual activity | Achaetomiella gracilis | |
Tween-20 | 5 mM, 2 h, 90% residual activity | Achaetomiella gracilis | |
Tween-80 | 5 mM, 2 h, 67% residual activity | Achaetomiella gracilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Achaetomiella gracilis | - |
- |
- |
Achaetomiella gracilis CGMCC3 3783 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Achaetomiella gracilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12569 | - |
pH 6.0, 55°C | Achaetomiella gracilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dextran T20 + H2O | - |
Achaetomiella gracilis | ? | - |
? | |
dextran T20 + H2O | - |
Achaetomiella gracilis CGMCC3 3783 | ? | - |
? | |
dextran T2000 + H2O | - |
Achaetomiella gracilis | ? | - |
? | |
dextran T2000 + H2O | - |
Achaetomiella gracilis CGMCC3 3783 | ? | - |
? | |
dextran T500 + H2O | - |
Achaetomiella gracilis | ? | - |
? | |
dextran T500 + H2O | - |
Achaetomiella gracilis CGMCC3 3783 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Achaetomiella gracilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
rapid loss of activity above | Achaetomiella gracilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
2 h, 90% residual activity | Achaetomiella gracilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Achaetomiella gracilis |