Activating Compound | Comment | Organism | Structure |
---|---|---|---|
SDS | 10 mM, 115% of initial activity | Talaromyces pinophilus | |
Urea | 1 mM, 115% of initial activity | Talaromyces pinophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 5 mM, 3% residual activity | Talaromyces pinophilus | |
EDTA | 5 mM, 76% residual activity | Talaromyces pinophilus | |
Fe3+ | 5 mM, 77% residual activity | Talaromyces pinophilus | |
Tris/HCl | 5 mM, 2% residual activity | Talaromyces pinophilus | |
Zn2+ | 5 mM, 18% residual activity | Talaromyces pinophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
dextran T100 | pH 6.0, 45°C | Talaromyces pinophilus | |
0.063 | - |
dextran T70 | pH 6.0, 45°C | Talaromyces pinophilus | |
0.09 | - |
dextran T40 | pH 6.0, 45°C | Talaromyces pinophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Talaromyces pinophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Talaromyces pinophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14894 | - |
pH 6.0, 45°C | Talaromyces pinophilus |
Storage Stability | Organism |
---|---|
-18°C, 20 mM acetate, pH 6.0, stable for 56 days | Talaromyces pinophilus |
4°C, 20 mM acetate, pH 6.0, stable for 56 days | Talaromyces pinophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dextran T100 + H2O | - |
Talaromyces pinophilus | isomaltose + isomaltotriose + isomaltooligosaccharides | - |
? | |
dextran T40 + H2O | - |
Talaromyces pinophilus | isomaltose + isomaltotriose + isomaltooligosaccharides | - |
? | |
dextran T70 + H2O | - |
Talaromyces pinophilus | isomaltose + isomaltotriose + isomaltooligosaccharides | - |
? | |
additional information | no activity towards beta-cyclodextrin, chitin, Sepharose 6B, chitosan, and sucrose. Low activity towards soluble starch | Talaromyces pinophilus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 58000, SDS-PAGE | Talaromyces pinophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Talaromyces pinophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
10 h, pH 6.0, almost no loss of activity | Talaromyces pinophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
163 | - |
dextran T40 | pH 6.0, 45°C | Talaromyces pinophilus | |
369 | - |
dextran T70 | pH 6.0, 45°C | Talaromyces pinophilus | |
4872 | - |
dextran T100 | pH 6.0, 45°C | Talaromyces pinophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Talaromyces pinophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 10 | - |
Talaromyces pinophilus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1800 | - |
dextran T40 | pH 6.0, 45°C | Talaromyces pinophilus | |
5810 | - |
dextran T70 | pH 6.0, 45°C | Talaromyces pinophilus | |
242400 | - |
dextran T100 | pH 6.0, 45°C | Talaromyces pinophilus |