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Literature summary for 3.2.1.11 extracted from

  • Sufiate, B.; Soares, F.; Moreira, S.; Gouveia, A.; Cardoso, E.; Braga, F.; de Araujo, J.; de Queiroz, J.
    In vitro and in silico characterization of a novel dextranase from Pochonia chlamydosporia (2018), 3 Biotech, 8, 167 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.11

Crystallization (Commentary)

Crystallization (Comment) Organism
molecular modeling of structure, based on PDB entry 1ogm.1 Pochonia chlamydosporia

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 10 mM, 17% residual activity Pochonia chlamydosporia
Ca2+ 10 mM, 77% residual activity Pochonia chlamydosporia
Cu2+ 10 mM, 51% residual activity Pochonia chlamydosporia
EDTA 10 mM, 80% residual activity Pochonia chlamydosporia
Fe2+ 10 mM, 71% residual activity Pochonia chlamydosporia
Hg2+ 10 mM, 17% residual activity Pochonia chlamydosporia
Mg2+ 10 mM, 60% residual activity Pochonia chlamydosporia
Mn2+ 10 mM, 89% residual activity Pochonia chlamydosporia
SDS 10 mM, 79% residual activity Pochonia chlamydosporia
Zn2+ 10 mM, 88% residual activity Pochonia chlamydosporia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 Dextran Km value 1.77 g/l, pH 6.0, 50°C, dextran with molecular weight from 60000 to 90000 Da Pochonia chlamydosporia

Organism

Organism UniProt Comment Textmining
Pochonia chlamydosporia A0A179FQV9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pochonia chlamydosporia

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
358
-
 pH 6.0, 50°C Pochonia chlamydosporia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dextran + H2O
-
 Pochonia chlamydosporia isomaltooligosaccharides
-
 ?

Subunits

Subunits Comment Organism
? x * 64000, SDS-PAGE, x * 64380, calculated Pochonia chlamydosporia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Pochonia chlamydosporia

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60
-
 48% of maximum activity Pochonia chlamydosporia

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 180 min, 48% resiudal activity Pochonia chlamydosporia
50
-
 30 min, 35% residual activity Pochonia chlamydosporia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Pochonia chlamydosporia

pH Range

pH Minimum pH Maximum Comment Organism
6
-
 more than 90% of maximum activity Pochonia chlamydosporia

pI Value

Organism Comment pI Value Maximum pI Value
Pochonia chlamydosporia 74% of maximum activity
-
 4
Pochonia chlamydosporia calculated
-
 6