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Literature summary for 3.2.1.11 extracted from
- Kinetics and thermodynamic of the purified dextranase from Chaetomium erraticum (2015), J. Mol. Catal. B, 122, 80-86.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 1 mM, 32% resiudal activity | Chaetomium erraticum |  |
| Fe3+ | 1 mM, 84% resiudal activity | Chaetomium erraticum | |
| Hg2+ | 1 mM, no resiudal activity | Chaetomium erraticum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.043 | - |
Dextran | pH 5.2, 30°C | Chaetomium erraticum | |
| 0.075 | - |
Dextran | pH 5.2, 40°C | Chaetomium erraticum | |
| 0.147 | - |
Dextran | pH 5.2, 50°C | Chaetomium erraticum | |
| 0.26 | - |
Dextran | pH 5.2, 60°C | Chaetomium erraticum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM, 117% of initial activity | Chaetomium erraticum | |
| Co2+ | 1 mM, 124% of initial activity | Chaetomium erraticum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59000 | - |
2 * 59000, SDS-PAGE | Chaetomium erraticum |
| 120000 | - |
PAGE | Chaetomium erraticum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chaetomium erraticum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Chaetomium erraticum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1106 | - |
pH 5.2, 60°C | Chaetomium erraticum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dextran + H2O | 100 kDa dextran | Chaetomium erraticum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 59000, SDS-PAGE | Chaetomium erraticum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Chaetomium erraticum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | 65 | more than 85% of maximum activity | Chaetomium erraticum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 723 | - |
Dextran | pH 5.2, 30°C | Chaetomium erraticum | |
| 1362 | - |
Dextran | pH 5.2, 40°C | Chaetomium erraticum | |
| 2865 | - |
Dextran | pH 5.2, 50°C | Chaetomium erraticum | |
| 4564 | - |
Dextran | pH 5.2, 60°C | Chaetomium erraticum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | - |
- |
Chaetomium erraticum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6 | more than 85% of maximum activity | Chaetomium erraticum |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 16800 | - |
Dextran | pH 5.2, 30°C | Chaetomium erraticum | |
| 17600 | - |
Dextran | pH 5.2, 60°C | Chaetomium erraticum | |
| 18300 | - |
Dextran | pH 5.2, 40°C | Chaetomium erraticum | |
| 19500 | - |
Dextran | pH 5.2, 50°C | Chaetomium erraticum | |
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