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Literature summary for 3.2.1.11 extracted from

  • Kim, Y.M.; Kiso, Y.; Muraki, T.; Kang, M.S.; Nakai, H.; Saburi, W.; Lang, W.; Kang, H.K.; Okuyama, M.; Mori, H.; Suzuki, R.; Funane, K.; Suzuki, N.; Momma, M.; Fujimoto, Z.; Oguma, T.; Kobayashi, M.; Kim, D.; Kimura, A.
    Novel dextranase catalyzing cycloisomaltooligosaccharide formation and identification of catalytic amino acids and their functions using chemical rescue approach (2012), J. Biol. Chem., 287, 19927-19935.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.11

Cloned(Commentary)

Cloned (Comment) Organism
-
 Paenibacillus sp.

Protein Variants

Protein Variants Comment Organism
D1254N 36% of wild-type activity Paenibacillus sp.
D189A application of achemical rescue approach using alpha-isomaltotetraosyl fluoride with NaN3. Mutant forms small sized dextran from alpha-isomaltotetraosyl fluoride in the presence of NaN3 Paenibacillus sp.
D340G application of a chemical rescue approach using alpha-isomaltotetraosyl fluoride with NaN3. Mutant forms beta-isomaltotetraosyl azide indicating residue D340 as nucleophile catalyst Paenibacillus sp.
E412Q application of a chemical rescue approach using alpha-isomaltotetraosyl fluoride with NaN3. Mutant forms alpha-isomaltotetraosyl azide indicating residue E412 as acid/base catalyst Paenibacillus sp.
additional information a C-terminal truncated enzyme, residues Ala39-Ser1304, has 50% wild-type activity Paenibacillus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000000183
-
 dextran T2000 pH 5.5, 35°C Paenibacillus sp.
0.000288
-
 isomaltoheptaose pH 5.5, 35°C Paenibacillus sp.
0.000386
-
 isomaltohexaose pH 5.5, 35°C Paenibacillus sp.
0.0004
-
 isomaltopentaose pH 5.5, 35°C Paenibacillus sp.

Organism

Organism UniProt Comment Textmining
Paenibacillus sp. H2EI27
-
-
Paenibacillus sp. Dex2000 H2EI27
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dextran + H2O
-
 Paenibacillus sp. isomaltotetraose + cycloisomaltooligosaccharides main products are isomaltotetraose and small amounts of cycloisomaltooligosaccharides with a degree of polymerization of 7-14 ?
dextran T2000 + H2O
-
 Paenibacillus sp. ?
-
 ?
isomaltoheptaose + H2O
-
 Paenibacillus sp. ?
-
 ?
isomaltoheptaose + H2O
-
 Paenibacillus sp. Dex2000 ?
-
 ?
isomaltohexaose + H2O
-
 Paenibacillus sp. ?
-
 ?
isomaltohexaose + H2O
-
 Paenibacillus sp. Dex2000 ?
-
 ?
isomaltopentaose + H2O
-
 Paenibacillus sp. ?
-
 ?
isomaltopentaose + H2O
-
 Paenibacillus sp. Dex2000 ?
-
 ?
additional information glycosyl hydrolase family 66 enzymes may be classified into three types: dextranases showing only dextranolytic activity, dextranases catalyzing hydrolysis with low cyclization activity, and cycloisomaltooligosaccharide glucanotransferases showing cycloisomaltooligosaccharide-forming activity with low dextranolytic activity Paenibacillus sp. ?
-
 ?
additional information no or very low activity on substrates isomaltose, isomaltotriose, isomaltotetraose Paenibacillus sp. ?
-
 ?
additional information glycosyl hydrolase family 66 enzymes may be classified into three types: dextranases showing only dextranolytic activity, dextranases catalyzing hydrolysis with low cyclization activity, and cycloisomaltooligosaccharide glucanotransferases showing cycloisomaltooligosaccharide-forming activity with low dextranolytic activity Paenibacillus sp. Dex2000 ?
-
 ?
additional information no or very low activity on substrates isomaltose, isomaltotriose, isomaltotetraose Paenibacillus sp. Dex2000 ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.36
-
 isomaltopentaose pH 5.5, 35°C Paenibacillus sp.
7.53
-
 isomaltohexaose pH 5.5, 35°C Paenibacillus sp.
7.93
-
 isomaltoheptaose pH 5.5, 35°C Paenibacillus sp.
44.3
-
 dextran T2000 pH 5.5, 35°C Paenibacillus sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
10500
-
 isomaltopentaose pH 5.5, 35°C Paenibacillus sp.
19500
-
 isomaltohexaose pH 5.5, 35°C Paenibacillus sp.
27500
-
 isomaltoheptaose pH 5.5, 35°C Paenibacillus sp.
242000000
-
 dextran T2000 pH 5.5, 35°C Paenibacillus sp.