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Literature summary for 3.2.1.11 extracted from

  • Esawy, M.; Mansour, S.; Ahmed, E.; Hassanein, N.; El Enshasy, H.
    Characterization of extracellular dextranase from a novel halophilic Bacillus subtilis NRC-B233b a mutagenic honey isolate under solid state fermentation (2012), E-J. Chem., 9, 1494-1510.
No PubMed abstract available

Application

Application Comment Organism
synthesis optimized culture conditions for dextranase productions are 37°C, pH 10, 32 h, and 20% (v/w) moisture content. The addition of 0.175 mM CrCl3 increases the enzyme production by about 4.5fold Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
NaCl 10%, 4fold decrease in activity Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis NRC-B233b
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O 165% of the activity with dextran Bacillus subtilis ?
-
?
amylopectin + H2O 165% of the activity with dextran Bacillus subtilis NRC-B233b ?
-
?
blue dextran + H2O 273% of the activity with dextran Bacillus subtilis ?
-
?
blue dextran + H2O 273% of the activity with dextran Bacillus subtilis NRC-B233b ?
-
?
dextran + H2O substrate dextran 250 kDa Bacillus subtilis oligosaccharides
-
?
dextran + H2O substrate dextran 250 kDa Bacillus subtilis NRC-B233b oligosaccharides
-
?
inulin + H2O 170% of the activity with dextran Bacillus subtilis ?
-
?
inulin + H2O 170% of the activity with dextran Bacillus subtilis NRC-B233b ?
-
?
starch + H2O 228% of the activity with dextran Bacillus subtilis ?
-
?
starch + H2O 228% of the activity with dextran Bacillus subtilis NRC-B233b ?
-
?
sucrose + H2O 100% of the activity with dextran Bacillus subtilis ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
1 h, no loss of activity Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.2
-
-
Bacillus subtilis