Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudothermotoga lettingae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 10% residual activity at 1 mM Ag+ | Pseudothermotoga lettingae | |
Fe2+ | 10% residual activity at1 mM Fe2+ | Pseudothermotoga lettingae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0095 | - |
dextran T2000 | in 40 mM sodium acetate buffer (pH4.3) at 55°C | Pseudothermotoga lettingae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activity increases to 125% in the presence of 1 mM Mg2+ | Pseudothermotoga lettingae | |
additional information | Ca2+, Li+, K+, and Na+ (with chloride) do not show appreciable effects on activity | Pseudothermotoga lettingae | |
Ni2+ | activity increases to 125% in the presence of 1 mM Ni2+ | Pseudothermotoga lettingae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64000 | - |
SDS-PAGE | Pseudothermotoga lettingae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alternan + H2O | Pseudothermotoga lettingae | 15% activity compared to dextran T70 | ? | - |
? | |
alternan + H2O | Pseudothermotoga lettingae TMO | 15% activity compared to dextran T70 | ? | - |
? | |
dextran T10 + H2O | Pseudothermotoga lettingae | 73% activity compared to dextran T70 | isomaltose + isomaltotriose | - |
? | |
dextran T10 + H2O | Pseudothermotoga lettingae TMO | 73% activity compared to dextran T70 | isomaltose + isomaltotriose | - |
? | |
dextran T2000 + H2O | Pseudothermotoga lettingae | 96% activity compared to dextran T70, the enzyme activity is very specific for alpha-(1-6)-glucosidic glucan | isomaltose + isomaltotriose | - |
? | |
dextran T2000 + H2O | Pseudothermotoga lettingae TMO | 96% activity compared to dextran T70, the enzyme activity is very specific for alpha-(1-6)-glucosidic glucan | isomaltose + isomaltotriose | - |
? | |
dextran T40 + H2O | Pseudothermotoga lettingae | 88% activity compared to dextran T70 | isomaltose + isomaltotriose | - |
? | |
dextran T500 + H2O | Pseudothermotoga lettingae | 96% activity compared to dextran T70 | isomaltose + isomaltotriose | - |
? | |
dextran T70 + H2O | Pseudothermotoga lettingae | 100% activity | isomaltose + isomaltotriose | - |
? | |
additional information | Pseudothermotoga lettingae | the enzyme cannot hydrolyze maltose, maltotriose, kojibiose, nigerose, sucrose, methyl alpha-glucoside, trehalose, soluble starch, and panose | ? | - |
? | |
additional information | Pseudothermotoga lettingae TMO | the enzyme cannot hydrolyze maltose, maltotriose, kojibiose, nigerose, sucrose, methyl alpha-glucoside, trehalose, soluble starch, and panose | ? | - |
? | |
pullulan + H2O | Pseudothermotoga lettingae | 4% activity compared to dextran T70 | ? | - |
? | |
pullulan + H2O | Pseudothermotoga lettingae TMO | 4% activity compared to dextran T70 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudothermotoga lettingae | A8F6K5 | - |
- |
Pseudothermotoga lettingae TMO | A8F6K5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alternan + H2O | 15% activity compared to dextran T70 | Pseudothermotoga lettingae | ? | - |
? | |
alternan + H2O | 15% activity compared to dextran T70 | Pseudothermotoga lettingae TMO | ? | - |
? | |
dextran T10 + H2O | 73% activity compared to dextran T70 | Pseudothermotoga lettingae | isomaltose + isomaltotriose | - |
? | |
dextran T10 + H2O | 73% activity compared to dextran T70 | Pseudothermotoga lettingae TMO | isomaltose + isomaltotriose | - |
? | |
dextran T2000 + H2O | 96% activity compared to dextran T70, the enzyme activity is very specific for alpha-(1-6)-glucosidic glucan | Pseudothermotoga lettingae | isomaltose + isomaltotriose | - |
? | |
dextran T2000 + H2O | 96% activity compared to dextran T70, the enzyme activity is very specific for alpha-(1-6)-glucosidic glucan | Pseudothermotoga lettingae TMO | isomaltose + isomaltotriose | - |
? | |
dextran T40 + H2O | 88% activity compared to dextran T70 | Pseudothermotoga lettingae | isomaltose + isomaltotriose | - |
? | |
dextran T500 + H2O | 96% activity compared to dextran T70 | Pseudothermotoga lettingae | isomaltose + isomaltotriose | - |
? | |
dextran T70 + H2O | 100% activity | Pseudothermotoga lettingae | isomaltose + isomaltotriose | - |
? | |
additional information | the enzyme cannot hydrolyze maltose, maltotriose, kojibiose, nigerose, sucrose, methyl alpha-glucoside, trehalose, soluble starch, and panose | Pseudothermotoga lettingae | ? | - |
? | |
additional information | the enzyme cannot hydrolyze maltose, maltotriose, kojibiose, nigerose, sucrose, methyl alpha-glucoside, trehalose, soluble starch, and panose | Pseudothermotoga lettingae TMO | ? | - |
? | |
pullulan + H2O | 4% activity compared to dextran T70 | Pseudothermotoga lettingae | ? | - |
? | |
pullulan + H2O | 4% activity compared to dextran T70 | Pseudothermotoga lettingae TMO | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TLDex | recombinant protein | Pseudothermotoga lettingae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 60 | for 15 min incubation | Pseudothermotoga lettingae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 65 | - |
Pseudothermotoga lettingae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 65 | over 75% of the original activity is sustained at 60°C for 4 h, the half-life of the enzyme is 1.5 h at 65°C with pH4.3 in the absence of added dextran | Pseudothermotoga lettingae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.3 | - |
- |
Pseudothermotoga lettingae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.5 | 4.5 | - |
Pseudothermotoga lettingae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.3 | 10 | stable from pH 4.3-10.0 after incubation at 4°C for 20 h, activity drops sharply above pH 10.0 and below pH 4.3 | Pseudothermotoga lettingae |