Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae INVSc1 via fusion of the DNA segment encoding the mature dextranase protein with alpha-factor signal sequence, and insertion into the GAL1-controlled expression vector pYES2/CT (construction of the four dextranase-expressing plasmids pYES2/CT-dex, pYES2/CT-dex-tag, pYES2/CT-alphaF-dex(m) and pYES2/CT-alphaF-dex(m)-tag). Dextranase construct pYESCT2-alphaF-dex(m) introduced into a strain of Saccharomyces cerevisiae expressing the human cytochrome P4503A4 (CYP3A4) and the cognate reductase | Talaromyces minioluteus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
aflatoxin B1 | inhibits dextranase activities of CYP3A4-expressing cultures, maximum inhibition of 22% at 2 microg/ml. Only negligibly inhibits strain INVSC1 expressing dextranase | Talaromyces minioluteus | |
aflatoxin G1 | substantially less inhibitory towards dextranase activities of CYP3A4-expressing cultures than aflatoxin B1, showing a maximum inhibition of about 12% at 2 microg/ml, whereas strain INVSC1 expressing dextranase is only marginally inhibited | Talaromyces minioluteus | |
additional information | no significant inhibition of dextranase activities of CYP3A4-expressing cultures and strain INVSC1 at concentrations of 2, 20, 200, and 2000 ng/ml by deoxynivalenol, fumonisin B1, and ochratoxin A | Talaromyces minioluteus | |
T-2 toxin | 100% inhibition at 10 microg/ml of dextranase activities of CYP3A4-expressing cultures and strain INVSC1 | Talaromyces minioluteus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Talaromyces minioluteus | Q27JJ3 | - |
- |
Talaromyces minioluteus IMI068219 | Q27JJ3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-sindacene-3-propionic acid, succinimidyl ester)-quenched dextran + H2O | - |
Talaromyces minioluteus | ? | - |
? | |
(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-sindacene-3-propionic acid, succinimidyl ester)-quenched dextran + H2O | - |
Talaromyces minioluteus IMI068219 | ? | - |
? |