Cloned (Comment) | Organism |
---|---|
wild-type dex1 gene directly cloned into NotI and XbaI sites of pEH75 and transformed into Escherichia coli Mach1TM-T1 cells. Dex1 coding regions from wild-type and thermostable mutants PCR-amplified from pEH75 and cloned into pET-45b(+) and transformed into Escherichia coli expression host BL21(DE3) | Paenibacillus sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | dexI mutants with improved thermostability. In terms of their relative specific activity towards dextran, noticeable differences among mutants showing increased, similar or reduced enzyme activity as compared to wild-type | Paenibacillus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus sp. | Q6VUG7 | - |
- |
Purification (Comment) | Organism |
---|---|
N-terminal His-tag purified enzyme | Paenibacillus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dextran + H2O | - |
Paenibacillus sp. | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
temperature optima of mutants are similar to that of the wild-type | Paenibacillus sp. |