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Literature summary for 3.2.1.11 extracted from

  • Hild, E.; Brumbley, S.M.; OShea, M.G.; Nevalainen, H.; Bergquist, P.L.
    A Paenibacillus sp. dextranase mutant pool with improved thermostability and activity (2007), Appl. Microbiol. Biotechnol., 75, 1071-1078.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
wild-type dex1 gene directly cloned into NotI and XbaI sites of pEH75 and transformed into Escherichia coli Mach1TM-T1 cells. Dex1 coding regions from wild-type and thermostable mutants PCR-amplified from pEH75 and cloned into pET-45b(+) and transformed into Escherichia coli expression host BL21(DE3) Paenibacillus sp.

Protein Variants

Protein Variants Comment Organism
additional information dexI mutants with improved thermostability. In terms of their relative specific activity towards dextran, noticeable differences among mutants showing increased, similar or reduced enzyme activity as compared to wild-type Paenibacillus sp.

Organism

Organism UniProt Comment Textmining
Paenibacillus sp. Q6VUG7
-
-

Purification (Commentary)

Purification (Comment) Organism
N-terminal His-tag purified enzyme Paenibacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dextran + H2O
-
Paenibacillus sp. ?
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?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
temperature optima of mutants are similar to that of the wild-type Paenibacillus sp.