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Literature summary for 3.2.1.11 extracted from
- Purification and partial characterization of the multicomponent dextranase complex of Streptococcus sobrinus and cloning of the dextranase gene (1987), Infect. Immun., 55, 792-802.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | plays a role in dental caries | Streptococcus sobrinus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Streptococcus sobrinus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.1 | - |
Dextran T-2000 | 175000-molecular-weight dextranase | Streptococcus sobrinus | |
| 1.25 | - |
Dextran T-2000 | 160000-molecular-weight dextranase | Streptococcus sobrinus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 160000 | - |
SDS-PAGE and gel filtration | Streptococcus sobrinus |
| 175000 | - |
SDS-PAGE and gel filtration | Streptococcus sobrinus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptococcus sobrinus | - |
- |
- |
| Streptococcus sobrinus UAB66 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptococcus sobrinus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10580 | - |
160000-molecular-weight dextranase | Streptococcus sobrinus |
| 14500 | - |
175000-molecular-weight dextranase | Streptococcus sobrinus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dextran T-2000 + H2O | - |
Streptococcus sobrinus | ? | - |
? |  |
| dextran T-2000 + H2O | - |
Streptococcus sobrinus UAB66 | ? | - |
? | |
| glucan + H2O | - |
Streptococcus sobrinus | ? | - |
? | |
| glucan + H2O | - |
Streptococcus sobrinus UAB66 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| multimer | - |
Streptococcus sobrinus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 36 | - |
- |
Streptococcus sobrinus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | - |
160000-molecular-weight dextranase | Streptococcus sobrinus |
| 5.4 | - |
175000-molecular-weight dextranase | Streptococcus sobrinus |
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Release 2023.1 (January 2023)
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