Literature summary for 3.2.1.108 extracted from

Beau, I.; Cotte-Laffitte, J.; Geniteau-Legendre, M.; Estes, M.K.; Servin, A.L.
An NSP4-dependant mechanism by which rotavirus impairs lactase enzymatic activity in brush border of human enterocyte-like Caco-2 cells (2007), Cell. Microbiol., 9, 2254-2266.

Search for more literature for 3.2.1.108

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	rotavirus impairs lactase enzymatic activity by an NSP4-dependant mechanism in brush border of human enterocyte-like Caco-2 cells, the infected brush border membranes show an enzyme with altered kinetics, overview	Homo sapiens
NSP4	impairment of lactase activity observed during rotavirus infection results from an inhibitory action of the secreted non-structural rotavirus protein NSP4	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	rotavirus infection decreases Vmax of LPH, overview	Homo sapiens
28.9	-	lactose	PPH from RRV-infected cells	Homo sapiens
30	-	lactose	LPH from uninfected cells	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
brush border	-	Homo sapiens	5903	-
brush border	associated	Homo sapiens	5903	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
130000	-	molecular mass after N-glycosidase F treatment of LPH	Homo sapiens
130000	-	N-glycosylated LPH, gel filtration	Homo sapiens
160000	-	cleaved intestinal mature form of LPH, determinated by pulse-chase experiments	Homo sapiens
160000	-	N- and O-glycosylated LPH, gel filtration	Homo sapiens
220000	-	apparent molecular mass of the high-mannose LPH form, determinated by pulse-chase experiments	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
lactose + H2O	Homo sapiens	rotavirus RRV infection affects the lactose metabolism of enterocytes in the intestine, overview	D-glucose + D-galactose	-	?
lactose + H2O	Homo sapiens	-	D-galactose + D-glucose	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	existence of two forms of brush border membrane-associated LPH, as N-glycosylated molecule and N- and O-glycosylated molecule	Homo sapiens
glycoprotein	two forms of brush border membrane-associated LPH are known, an N-glycosylated, and an N- and O-glycosylated molecule	Homo sapiens
proteolytic modification	human LPH is synthesized as a precursor pro-LPH molecule, pro-LPH undergoes proteolytic cleavage to yield mature LPH by a trypsin-like protease that occurs intracellularly	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
Caco-2 cell	-	Homo sapiens	-
Caco-2 cell	fully differentiated, intestinal enterocyte-like cells	Homo sapiens	-
enterocyte	predominantly at the apical membrane	Homo sapiens	-
intestine	intestinal cells that hydrolyse lactose	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.00164	-	non-infected cells, lactase enzyme activity in cell homogenates	Homo sapiens
0.00167	-	rhesus monkey rotavirus-, RRV, infected cells, lactase enzyme activity in cell homogenates	Homo sapiens
0.00219	-	rhesus monkey rotavirus-, RRV, infected cells, lactase enzyme activity in brush border-enriched fraction	Homo sapiens
0.00344	-	non-infected cells, lactase enzyme activity in brush border-enriched fraction	Homo sapiens
0.00359	-	UV, psoralen-inactivated RRV infected cells, lactase enzyme activity in brush border-enriched fraction	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
lactose + H2O	-	Homo sapiens	D-glucose + D-galactose	-	?
lactose + H2O	rotavirus RRV infection affects the lactose metabolism of enterocytes in the intestine, overview	Homo sapiens	D-glucose + D-galactose	-	?
lactose + H2O	-	Homo sapiens	D-galactose + D-glucose	-	?

Synonyms

Synonyms	Comment	Organism
lactase	-	Homo sapiens
lactase-phlorizin hydrolase	-	Homo sapiens
LPH	-	Homo sapiens

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Release 2023.1 (January 2023)