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Literature summary for 3.2.1.108 extracted from

  • Skovbjerg, H.; Sjöström, H.; Noren, O.
    Purification and characterization of amphiphilic lactase/phlorizin hydrolase from human small intestine (1981), Eur. J. Biochem., 114, 653-661.
    View publication on PubMed

General Stability

General Stability Organism
repeated freezing and thawing, activity declines fast Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
phlorizin
-
Homo sapiens
Tris competitive Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.44
-
phlorizin
-
Homo sapiens
4.4
-
cellobiose
-
Homo sapiens
21
-
lactose
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000
-
2 * 160000, SDS-PAGE Homo sapiens
280000
-
hydrophilic enzyme form, gel filtration Homo sapiens
320000
-
amphiphilic enzyme form, gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
small intestine
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
32
-
-
Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, stable for several months Homo sapiens
4°C, stable for several weeks Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + H2O 14% of the activity with lactose Homo sapiens 2 beta-D-glucose
-
?
cellulose + H2O
-
Homo sapiens beta-D-glucose
-
?
gentiobiose + H2O 0.7% of the activity with lactose Homo sapiens 2 beta-D-glucose
-
?
lactose + H2O
-
Homo sapiens D-glucose + D-galactose
-
?
o-nitrophenyl-beta-galactoside + H2O 17% of the activity with lactose Homo sapiens o-nitrophenol + beta-D-galactose
-
?
p-nitrophenyl-beta-galactoside + H2O 13% of the activity with lactose Homo sapiens p-nitrophenol + beta-D-galactose
-
?
p-nitrophenyl-beta-glucoside + H2O 10% of the activity with lactose Homo sapiens p-nitrophenol + beta-D-glucose
-
?
phenyl-beta-galactoside + H2O 0.1% of the activity with lactose Homo sapiens phenol + beta-D-galactose
-
?
phlorizin + H2O 22% of the activity with lactose Homo sapiens phloretin + D-glucose
-
?

Subunits

Subunits Comment Organism
dimer 2 * 160000, SDS-PAGE Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8 6 hydrolysis of lactose Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
5 7 pH 5.0: about 70% of maximal activity, pH 7.0: about 65% of maximal activity Homo sapiens