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Literature summary for 3.2.1.107 extracted from

  • Hamazaki, H.; Hamazaki, M.
    Catalytic site of human protein-glucosylgalactosylhydroxylysine glucosidase Three crucial carboxyl residues were determined by cloning and site-directed mutagenesis (2016), Biochem. Biophys. Res. Commun., 469, 357-362 .
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
ATHL1 cDNA is cloned to a cloning and expression vector and two plasmid clones with different ATHL1 CDS insert are obtained. Each plasmid DNA is transformed into Escherichia coli cells for expression and two isoforms of chicken PGGHG are obtained. Both isoforms effectively released glucose from type IV collagen Gallus gallus

Protein Variants

Protein Variants Comment Organism
D301E mutations leads to complete elimination of enzyme activity Gallus gallus
D301N mutations leads to complete elimination of enzyme activity Gallus gallus
E430D mutations leads to complete elimination of enzyme activity Gallus gallus
E430Q mutations leads to complete elimination of enzyme activity Gallus gallus
E574D mutations leads to complete elimination of enzyme activity Gallus gallus
E574Q mutations leads to complete elimination of enzyme activity Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus F1NZI4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
 Gallus gallus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Type IV collagen + H2O the enzyme cleaves glucose from disaccharide unit (Glc-alpha-(1,2)-Gal) linked to hydroxylysine residues of collagen Gallus gallus ?
-
 ?

Synonyms

Synonyms Comment Organism
ATHL1
-
 Gallus gallus
PGGHG
-
 Gallus gallus