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Literature summary for 3.2.1.107 extracted from
- Enzymatic hydrolysis of disaccharide unit of collagen. Isolation of 2-O-alpha-D-glucopyranosyl-O-beta-D-galactopyranosyl-hydroxylysine glucohydrolase from rat spleens (1980), Eur. J. Biochem., 111, 587-591.
General Stability
| General Stability | Organism |
|---|---|
| 15 mg/l bovine serum albumin stabilize | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Rattus norvegicus |  |
| glucosamine | competitive | Rattus norvegicus | |
| p-chloromercuribenzoate | 4 micromol complete inhibition | Rattus norvegicus | |
| Zn2+ | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.9 | - |
2-O-Alpha-D-glucopyranosyl-O-beta-D-galactopyranosylhydroxylysine | - |
Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 85000 | - |
SDS-PAGE | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
Sprague-Dawley | - |
| Rattus norvegicus Sprague-Dawley | - |
Sprague-Dawley | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
| heart | - |
Rattus norvegicus | - |
| intestine | - |
Rattus norvegicus | - |
| kidney | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
| lung | - |
Rattus norvegicus | - |
| spleen | - |
Rattus norvegicus | - |
| stomach | - |
Rattus norvegicus | - |
| testis | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.3 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-O-alpha-D-glucopyranosyl-O-beta-D-galactopyranosylhydroxylysine + H2O | - |
Rattus norvegicus | alpha-D-glucose + beta-D-galactopyranosyl-L-hydroxylysine | - |
? | |
| 2-O-alpha-D-glucopyranosyl-O-beta-D-galactopyranosylhydroxylysine + H2O | - |
Rattus norvegicus Sprague-Dawley | alpha-D-glucose + beta-D-galactopyranosyl-L-hydroxylysine | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | - |
- |
Rattus norvegicus |
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Release 2023.1 (January 2023)
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