Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain JM109 | Aspergillus brasiliensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-deoxynojirimycin | - |
Aspergillus brasiliensis | |
kojibiose | - |
Aspergillus brasiliensis | |
nigerose | - |
Aspergillus brasiliensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0042 | - |
Glcalpha(1->2)Glcalpha(1->3)Glcalpha(1->3)Manalpha(1->2)Manalpha(1->2)Manalpha(1->3)Manbeta(1->4)GlcNacbeta(1->4)GlcNacbeta-pyridylamine | in 20 mM sodium phosphate, pH 7.0, at 25°C | Aspergillus brasiliensis | |
0.0061 | - |
Glc3Man9GlcNAc2-pyridylamine | in 20 mM sodium phosphate, pH 7.0, at 25°C | Aspergillus brasiliensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus brasiliensis | G1UJA9 | formerly Aspergillus niger | - |
Aspergillus brasiliensis ATCC 9642 | G1UJA9 | formerly Aspergillus niger | - |
Purification (Comment) | Organism |
---|---|
glutathione-agarose column chromatography, gel filtration | Aspergillus brasiliensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Glc3Man9GlcNAc2-pyridylamine + H2O | - |
Aspergillus brasiliensis | Glc2Man9GlcNAc2-pyridylamine + D-glucose | - |
? | |
Glc3Man9GlcNAc2-pyridylamine + H2O | - |
Aspergillus brasiliensis ATCC 9642 | Glc2Man9GlcNAc2-pyridylamine + D-glucose | - |
? | |
Glcalpha(1->2)Glcalpha(1->3)Glcalpha(1->3)Manalpha(1->2)Manalpha(1->2)Manalpha(1->3)Manbeta(1->4)GlcNacbeta(1->4)GlcNacbeta-pyridylamine + H2O | - |
Aspergillus brasiliensis | Glcalpha(1->3)Glcalpha(1->3)Manalpha(1->2)Manalpha(1->2)Manalpha(1->3)Manbeta(1->4)GlcNacbeta(1->4)GlcNacbeta-pyridylamine + D-glucose | - |
? | |
Glcalpha(1->2)Glcalpha(1->3)Glcalpha(1->3)Manalpha(1->2)Manalpha(1->2)Manalpha(1->3)Manbeta(1->4)GlcNacbeta(1->4)GlcNacbeta-pyridylamine + H2O | - |
Aspergillus brasiliensis ATCC 9642 | Glcalpha(1->3)Glcalpha(1->3)Manalpha(1->2)Manalpha(1->2)Manalpha(1->3)Manbeta(1->4)GlcNacbeta(1->4)GlcNacbeta-pyridylamine + D-glucose | - |
? | |
additional information | the enzyme does not hydrolyze the terminal alpha(1-2)-linkage of Glc2Man9GlcNAc2-pyridylamine | Aspergillus brasiliensis | ? | - |
? | |
additional information | the enzyme does not hydrolyze the terminal alpha(1-2)-linkage of Glc2Man9GlcNAc2-pyridylamine | Aspergillus brasiliensis ATCC 9642 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PGI | - |
Aspergillus brasiliensis |
processing alpha-glucosidase I | - |
Aspergillus brasiliensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1100 | - |
Glcalpha(1->2)Glcalpha(1->3)Glcalpha(1->3)Manalpha(1->2)Manalpha(1->2)Manalpha(1->3)Manbeta(1->4)GlcNacbeta(1->4)GlcNacbeta-pyridylamine | in 20 mM sodium phosphate, pH 7.0, at 25°C | Aspergillus brasiliensis | |
1450 | - |
Glc3Man9GlcNAc2-pyridylamine | in 20 mM sodium phosphate, pH 7.0, at 25°C | Aspergillus brasiliensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Aspergillus brasiliensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | the enzyme retains 89% and 94% of maximum activity at pH 6.0 and 8.0, respectively | Aspergillus brasiliensis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.02 | - |
in 20 mM sodium phosphate, pH 7.0, at 25°C | Aspergillus brasiliensis | 1-deoxynojirimycin | |
0.02 | - |
in 20 mM sodium phosphate, pH 7.0, at 25°C | Aspergillus brasiliensis | kojibiose | |
1.9 | - |
in 20 mM sodium phosphate, pH 7.0, at 25°C | Aspergillus brasiliensis | nigerose |