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Literature summary for 3.2.1.106 extracted from

  • Miyazaki, T.; Matsumoto, Y.; Matsuda, K.; Kurakata, Y.; Matsuo, I.; Ito, Y.; Nishikawa, A.; Tonozuka, T.
    Heterologous expression and characterization of processing alpha-glucosidase I from Aspergillus brasiliensis ATCC 9642 (2011), Glycoconj. J., 28, 563-571.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain JM109 Aspergillus brasiliensis

Inhibitors

Inhibitors Comment Organism Structure
1-deoxynojirimycin
-
Aspergillus brasiliensis
kojibiose
-
Aspergillus brasiliensis
nigerose
-
Aspergillus brasiliensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0042
-
Glcalpha(1->2)Glcalpha(1->3)Glcalpha(1->3)Manalpha(1->2)Manalpha(1->2)Manalpha(1->3)Manbeta(1->4)GlcNacbeta(1->4)GlcNacbeta-pyridylamine in 20 mM sodium phosphate, pH 7.0, at 25°C Aspergillus brasiliensis
0.0061
-
Glc3Man9GlcNAc2-pyridylamine in 20 mM sodium phosphate, pH 7.0, at 25°C Aspergillus brasiliensis

Organism

Organism UniProt Comment Textmining
Aspergillus brasiliensis G1UJA9 formerly Aspergillus niger
-
Aspergillus brasiliensis ATCC 9642 G1UJA9 formerly Aspergillus niger
-

Purification (Commentary)

Purification (Comment) Organism
glutathione-agarose column chromatography, gel filtration Aspergillus brasiliensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Glc3Man9GlcNAc2-pyridylamine + H2O
-
Aspergillus brasiliensis Glc2Man9GlcNAc2-pyridylamine + D-glucose
-
?
Glc3Man9GlcNAc2-pyridylamine + H2O
-
Aspergillus brasiliensis ATCC 9642 Glc2Man9GlcNAc2-pyridylamine + D-glucose
-
?
Glcalpha(1->2)Glcalpha(1->3)Glcalpha(1->3)Manalpha(1->2)Manalpha(1->2)Manalpha(1->3)Manbeta(1->4)GlcNacbeta(1->4)GlcNacbeta-pyridylamine + H2O
-
Aspergillus brasiliensis Glcalpha(1->3)Glcalpha(1->3)Manalpha(1->2)Manalpha(1->2)Manalpha(1->3)Manbeta(1->4)GlcNacbeta(1->4)GlcNacbeta-pyridylamine + D-glucose
-
?
Glcalpha(1->2)Glcalpha(1->3)Glcalpha(1->3)Manalpha(1->2)Manalpha(1->2)Manalpha(1->3)Manbeta(1->4)GlcNacbeta(1->4)GlcNacbeta-pyridylamine + H2O
-
Aspergillus brasiliensis ATCC 9642 Glcalpha(1->3)Glcalpha(1->3)Manalpha(1->2)Manalpha(1->2)Manalpha(1->3)Manbeta(1->4)GlcNacbeta(1->4)GlcNacbeta-pyridylamine + D-glucose
-
?
additional information the enzyme does not hydrolyze the terminal alpha(1-2)-linkage of Glc2Man9GlcNAc2-pyridylamine Aspergillus brasiliensis ?
-
?
additional information the enzyme does not hydrolyze the terminal alpha(1-2)-linkage of Glc2Man9GlcNAc2-pyridylamine Aspergillus brasiliensis ATCC 9642 ?
-
?

Synonyms

Synonyms Comment Organism
PGI
-
Aspergillus brasiliensis
processing alpha-glucosidase I
-
Aspergillus brasiliensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1100
-
Glcalpha(1->2)Glcalpha(1->3)Glcalpha(1->3)Manalpha(1->2)Manalpha(1->2)Manalpha(1->3)Manbeta(1->4)GlcNacbeta(1->4)GlcNacbeta-pyridylamine in 20 mM sodium phosphate, pH 7.0, at 25°C Aspergillus brasiliensis
1450
-
Glc3Man9GlcNAc2-pyridylamine in 20 mM sodium phosphate, pH 7.0, at 25°C Aspergillus brasiliensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Aspergillus brasiliensis

pH Range

pH Minimum pH Maximum Comment Organism
6 8 the enzyme retains 89% and 94% of maximum activity at pH 6.0 and 8.0, respectively Aspergillus brasiliensis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.02
-
in 20 mM sodium phosphate, pH 7.0, at 25°C Aspergillus brasiliensis 1-deoxynojirimycin
0.02
-
in 20 mM sodium phosphate, pH 7.0, at 25°C Aspergillus brasiliensis kojibiose
1.9
-
in 20 mM sodium phosphate, pH 7.0, at 25°C Aspergillus brasiliensis nigerose