Cloned (Comment) | Organism |
---|---|
yeast strains transformed with pHVX2-His carrying truncated forms of CWH41. Mutated plasmids pE580A and pD584A transformed into Escherichia coli DH5-alpha and when transformed into Saccharomyces cerevisiae AH22 for expression | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
D584A | Cwht1p mutant, expressed at significantly lower concentrations than the parental Cwht1p, expressed at a lower concentration than mutant E580A, is inactive | Saccharomyces cerevisiae |
E580A | Cwht1p mutant, expressed at significantly lower concentrations than the parental Cwht1p, is inactive | Saccharomyces cerevisiae |
additional information | truncated form Cwht1p (E35-F833), with deletion of the N-terminus and transmembrane domain expressed as a catalytically active fragment while truncated forms Cwht2p (R320-F833) and Cwht3p (M526-F833) not detectable | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
94000 | - |
x * 94000, truncated form Cwht1p purified as a soluble non-glycosylated protein, SDS-PAGE | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae AH22 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
truncated form Cwht1p purified as a soluble non-glycosylated protein to approximately 90% homogeneity by a combination of ammonium sulfate precipitation, anion-exchange chromatography and immobilized metal affinity chromatography | Saccharomyces cerevisiae |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
140 | - |
Cwht1p | Saccharomyces cerevisiae |
3000 | - |
soluble alpha-glucosidase I | Saccharomyces cerevisiae |
3600 | - |
Cwht1p, purified 7.4fold | Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-80°C | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-Glc-(1-2)-alpha-D-Glc-(1-3)-alpha-D-Glc-O-(CH2)8COOCH3 | residues E613 and D617 play a crucial role in maintaining enzyme activity | Saccharomyces cerevisiae | ? | - |
? | |
alpha-D-Glc-(1-2)-alpha-D-Glc-(1-3)-alpha-D-Glc-O-(CH2)8COOCH3 | residues E613 and D617 play a crucial role in maintaining enzyme activity | Saccharomyces cerevisiae AH22 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 94000, truncated form Cwht1p purified as a soluble non-glycosylated protein, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
alpha-glucosidase I | - |
Saccharomyces cerevisiae |
Cwh41p | - |
Saccharomyces cerevisiae |
mannosyl-oligosaccharide glucosidase | - |
Saccharomyces cerevisiae |
processing alpha-glucosidase I | - |
Saccharomyces cerevisiae |