Literature summary for 3.2.1.106 extracted from

Dhanawansa, R.; Faridmoayer, A.; Van der Merwe, G.; Li, Y.X.; Scaman, C.H.
Overexpression, purification, and partial characterization of Saccharomyces cerevisiae processing alpha glucosidase I (2002), Glycobiology, 12, 229-234.

Search for more literature for 3.2.1.106

Inhibitors

Inhibitors	Comment	Organism	Structure
diethyl dicarbonate	55% inhibition at 1 mM after 30 min	Saccharomyces cerevisiae
ethyl-3-(3-(dimethylamino)propyl)carbodiimide	90% inhibition at 50 mM after 60 min at pH 6.8, 4°C, preincubation with deoxynorjirmycin prevents	Saccharomyces cerevisiae
kojibiose	-	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	proteolytic cleavage of the transmembrane form between residues Ala24 and Thr25 results in a soluble form	Saccharomyces cerevisiae	16020	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
96510	-	calculated from amino acid sequence	Saccharomyces cerevisiae
98000	-	SDS-PAGE	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-
Saccharomyces cerevisiae AH22	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	4 potential N-glycosylation sites	Saccharomyces cerevisiae

Purification (Commentary)

Purification (Comment)	Organism
soluble form	Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
8550	-	-	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
alpha-D-Glc-(1-2)-alpha-D-Glc-(1-3)-alpha-D-Glc-O(CH2)8-COOCH3 + H2O	synthetic substrate	Saccharomyces cerevisiae	beta-D-glucose + alpha-D-Glc-(1-3)-alpha-D-Glc-O(CH2)8-COOCH3	-	?
alpha-D-Glc-(1-2)-alpha-D-Glc-(1-3)-alpha-D-Glc-O(CH2)8-COOCH3 + H2O	synthetic substrate	Saccharomyces cerevisiae AH22	beta-D-glucose + alpha-D-Glc-(1-3)-alpha-D-Glc-O(CH2)8-COOCH3	-	?
Glc3Man9GlcNAc2 + H2O	3 glucose residues are required for catalysis	Saccharomyces cerevisiae	D-glucose + Glc2Man9GlcNAc2	-	?
Glc3Man9GlcNAc2 + H2O	3 glucose residues are required for catalysis	Saccharomyces cerevisiae AH22	D-glucose + Glc2Man9GlcNAc2	-	?

Synonyms

Synonyms	Comment	Organism
CWH41	-	Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.8	-	kojibiose	pH 6.8, 37°C	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)