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Literature summary for 3.2.1.106 extracted from

  • Kalz-Fuller, B.; Bieberich, E.; Bause, E.
    Cloning and expression of glucosidase I from human hippocampus (1995), Eur. J. Biochem., 231, 344-351.
    View publication on PubMed
Search for more literature for 3.2.1.106

Cloned(Commentary)

Cloned (Comment) Organism
cDNA, overexpression in COS 1 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1-deoxynojirimycin strong inhibition Homo sapiens
1-deoxynojirimycin strong inhibition Sus scrofa
additional information no inhibition by 1-deoxymannojirimycin Homo sapiens
additional information no inhibition by 1-deoxymannojirimycin Sus scrofa
N,N-dimethyl-1-deoxynojirimycin specific inhibitor; strong inhibition Homo sapiens
N,N-dimethyl-1-deoxynojirimycin specific inhibitor; strong inhibition Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum endoplasmic reticulum-resident glycoprotein Homo sapiens 5783
-
endoplasmic reticulum endoplasmic reticulum-resident glycoprotein Sus scrofa 5783
-
endoplasmic reticulum catalytic site/active centre located at lumenal side of endoplasmic reticulum Homo sapiens 5783
-
endoplasmic reticulum catalytic site/active centre located at lumenal side of endoplasmic reticulum Sus scrofa 5783
-
membrane membrane-bound Homo sapiens 16020
-
membrane membrane-bound Sus scrofa 16020
-
membrane type-II transmembrane protein Homo sapiens 16020
-
membrane type-II transmembrane protein Sus scrofa 16020
-
microsome
-
 Homo sapiens
-
-
microsome
-
 Sus scrofa
-
-
additional information subcellular localization Homo sapiens
-
-
additional information subcellular localization Sus scrofa
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information metal ions not required Homo sapiens
additional information metal ions not required Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
92000
-
 calculated from nucleotide sequence Homo sapiens
93000
-
 enzyme of 95000 Da degraded by endoglycosidase H, SDS-PAGE Homo sapiens
93000
-
 enzyme of 95000 Da degraded by endoglycosidase H, SDS-PAGE Sus scrofa
95000
-
 SDS-PAGE Homo sapiens
95000
-
 SDS-PAGE Sus scrofa
95000
-
 cDNA cloned, expressed in COS 1 cells, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Glc3Man9GlcNAc2 + H2O Homo sapiens hydrolyses specifically terminal alpha1,2-linked glucose residue ?
-
 ?
Glc3Man9GlcNAc2 + H2O Sus scrofa hydrolyses specifically terminal alpha1,2-linked glucose residue ?
-
 ?
Glc3Man9GlcNAc2 + H2O Homo sapiens specific involvement in the N-linked oligosaccharide-processing pathway ?
-
 ?
Glc3Man9GlcNAc2 + H2O Sus scrofa specific involvement in the N-linked oligosaccharide-processing pathway ?
-
 ?
Glc3Man9GlcNAc2 + H2O Homo sapiens involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
 ?
Glc3Man9GlcNAc2 + H2O Sus scrofa involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
 ?
Glc3Man9GlcNAc2 + H2O Homo sapiens involved in processing of asparagine-linked oligosaccharides ?
-
 ?
Glc3Man9GlcNAc2 + H2O Sus scrofa involved in processing of asparagine-linked oligosaccharides ?
-
 ?
additional information Homo sapiens involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
 ?
additional information Sus scrofa involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
 ?
additional information Homo sapiens involved in processing of asparagine-linked oligosaccharides ?
-
 ?
additional information Sus scrofa involved in processing of asparagine-linked oligosaccharides ?
-
 ?
additional information Homo sapiens involved in the formation of high mannose and complex glycoproteins ?
-
 ?
additional information Sus scrofa involved in the formation of high mannose and complex glycoproteins ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Sus scrofa
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein single N-glycosylation site, N-glycosylation about 2-3 kDa Homo sapiens
glycoprotein single N-glycosylation site, N-glycosylation about 2-3 kDa Sus scrofa
glycoprotein with high-mannose oligosaccharides Homo sapiens
glycoprotein with high-mannose oligosaccharides Sus scrofa

Purification (Commentary)

Purification (Comment) Organism
-
 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
hippocampus
-
 Homo sapiens
-
liver
-
 Homo sapiens
-
liver
-
 Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Homo sapiens D-glucose + Glc2Man9GlcNAc2
-
 ?
Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Sus scrofa D-glucose + Glc2Man9GlcNAc2
-
 ?
Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Homo sapiens ?
-
 ?
Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Sus scrofa ?
-
 ?
Glc3Man9GlcNAc2 + H2O specific involvement in the N-linked oligosaccharide-processing pathway Homo sapiens ?
-
 ?
Glc3Man9GlcNAc2 + H2O specific involvement in the N-linked oligosaccharide-processing pathway Sus scrofa ?
-
 ?
Glc3Man9GlcNAc2 + H2O involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Homo sapiens ?
-
 ?
Glc3Man9GlcNAc2 + H2O involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Sus scrofa ?
-
 ?
Glc3Man9GlcNAc2 + H2O involved in processing of asparagine-linked oligosaccharides Homo sapiens ?
-
 ?
Glc3Man9GlcNAc2 + H2O involved in processing of asparagine-linked oligosaccharides Sus scrofa ?
-
 ?
additional information Glc1Man9GlcNAc2: not a substrate Homo sapiens ?
-
 ?
additional information Glc1Man9GlcNAc2: not a substrate Sus scrofa ?
-
 ?
additional information also acts, more slowly, on the corresponding glycolipids and glycopeptides Homo sapiens ?
-
 ?
additional information also acts, more slowly, on the corresponding glycolipids and glycopeptides Sus scrofa ?
-
 ?
additional information aryl-alpha-D-glucosides: not a substrate Homo sapiens ?
-
 ?
additional information aryl-alpha-D-glucosides: not a substrate Sus scrofa ?
-
 ?
additional information Glc2Man9GlcNAc2: not a substrate Homo sapiens ?
-
 ?
additional information Glc2Man9GlcNAc2: not a substrate Sus scrofa ?
-
 ?
additional information involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Homo sapiens ?
-
 ?
additional information involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Sus scrofa ?
-
 ?
additional information involved in processing of asparagine-linked oligosaccharides Homo sapiens ?
-
 ?
additional information involved in processing of asparagine-linked oligosaccharides Sus scrofa ?
-
 ?
additional information involved in the formation of high mannose and complex glycoproteins Homo sapiens ?
-
 ?
additional information involved in the formation of high mannose and complex glycoproteins Sus scrofa ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
-
 Sus scrofa