Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | several-fold activation | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-deoxynojirimycin | strong inhibition | Bos taurus | |
N,N-dimethyl-1-deoxynojirimycin | strong inhibition | Bos taurus | |
N-(5-carboxypentyl)-1-deoxynojirimycin | slight inhibition | Bos taurus | |
N-dodecyl-1-deoxynojirimycin | slight inhibition | Bos taurus | |
N-methyl-1-deoxynojirimycin | strong inhibition | Bos taurus | |
Nojirimycin | potent inhibitor | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Bos taurus | 5783 | - |
membrane | membrane-bound | Bos taurus | 16020 | - |
microsome | - |
Bos taurus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
85000 | - |
calf, 4 * 85000, SDS-PAGE | Bos taurus |
320000 | 350000 | calf, gel filtration | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Glc3Man9GlcNAc2 + H2O | Bos taurus | hydrolyses specifically terminal alpha1,2-linked glucose residue | ? | - |
? | |
Glc3Man9GlcNAc2 + H2O | Bos taurus | specific involvement in the N-linked oligosaccharide-processing pathway | ? | - |
? | |
Glc3Man9GlcNAc2 + H2O | Bos taurus | involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins | ? | - |
? | |
Glc3Man9GlcNAc2 + H2O | Bos taurus | involved in processing of asparagine-linked oligosaccharides | ? | - |
? | |
additional information | Bos taurus | involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins | ? | - |
? | |
additional information | Bos taurus | involved in processing of asparagine-linked oligosaccharides | ? | - |
? | |
additional information | Bos taurus | involved in the formation of high mannose and complex glycoproteins | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
calf | - |
Purification (Comment) | Organism |
---|---|
calf | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | calf | Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bos taurus |
Storage Stability | Organism |
---|---|
2-4°C, 0.8% Lubrol PX, 200 mM phosphate, pH 6.8, 3 weeks, 50% enzyme activity retained, activity lost rapidly on the removal of detergent | Bos taurus |
4°C, presence of detergent, 0.8% Lubrol PX, t1/2: 20 days | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Glc3Man9GlcNAc2 + H2O | hydrolyses specifically terminal alpha1,2-linked glucose residue | Bos taurus | D-glucose + Glc2Man9GlcNAc2 | - |
? | |
Glc3Man9GlcNAc2 + H2O | hydrolyses specifically terminal alpha1,2-linked glucose residue | Bos taurus | ? | - |
? | |
Glc3Man9GlcNAc2 + H2O | specific involvement in the N-linked oligosaccharide-processing pathway | Bos taurus | ? | - |
? | |
Glc3Man9GlcNAc2 + H2O | involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins | Bos taurus | ? | - |
? | |
Glc3Man9GlcNAc2 + H2O | involved in processing of asparagine-linked oligosaccharides | Bos taurus | ? | - |
? | |
additional information | Glc1Man9GlcNAc2: not a substrate | Bos taurus | ? | - |
? | |
additional information | also acts, more slowly, on the corresponding glycolipids and glycopeptides | Bos taurus | ? | - |
? | |
additional information | p-nitrophenyl-alpha-D-glucopyranoside: not a substrate | Bos taurus | ? | - |
? | |
additional information | aryl-alpha-D-glucosides: not a substrate | Bos taurus | ? | - |
? | |
additional information | Glc2Man9GlcNAc2: not a substrate | Bos taurus | ? | - |
? | |
additional information | involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins | Bos taurus | ? | - |
? | |
additional information | involved in processing of asparagine-linked oligosaccharides | Bos taurus | ? | - |
? | |
additional information | involved in the formation of high mannose and complex glycoproteins | Bos taurus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | calf, 4 * 85000, SDS-PAGE | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
- |
Bos taurus |