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Literature summary for 3.2.1.106 extracted from

  • Hettkamp, H.; Legler, G.; Bause, E.
    Purification by affinity chromatography of glucosidase I, an endoplasmic reticulum hydrolase involved in the processing of asparagine-linked oligosaccharides (1984), Eur. J. Biochem., 142, 85-90.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 several-fold activation Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
1-deoxynojirimycin strong inhibition Bos taurus
N,N-dimethyl-1-deoxynojirimycin strong inhibition Bos taurus
N-(5-carboxypentyl)-1-deoxynojirimycin slight inhibition Bos taurus
N-dodecyl-1-deoxynojirimycin slight inhibition Bos taurus
N-methyl-1-deoxynojirimycin strong inhibition Bos taurus
Nojirimycin potent inhibitor Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
Bos taurus 5783
-
membrane membrane-bound Bos taurus 16020
-
microsome
-
Bos taurus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000
-
calf, 4 * 85000, SDS-PAGE Bos taurus
320000 350000 calf, gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Glc3Man9GlcNAc2 + H2O Bos taurus hydrolyses specifically terminal alpha1,2-linked glucose residue ?
-
?
Glc3Man9GlcNAc2 + H2O Bos taurus specific involvement in the N-linked oligosaccharide-processing pathway ?
-
?
Glc3Man9GlcNAc2 + H2O Bos taurus involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
?
Glc3Man9GlcNAc2 + H2O Bos taurus involved in processing of asparagine-linked oligosaccharides ?
-
?
additional information Bos taurus involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins ?
-
?
additional information Bos taurus involved in processing of asparagine-linked oligosaccharides ?
-
?
additional information Bos taurus involved in the formation of high mannose and complex glycoproteins ?
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
calf
-

Purification (Commentary)

Purification (Comment) Organism
calf Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver calf Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Bos taurus

Storage Stability

Storage Stability Organism
2-4°C, 0.8% Lubrol PX, 200 mM phosphate, pH 6.8, 3 weeks, 50% enzyme activity retained, activity lost rapidly on the removal of detergent Bos taurus
4°C, presence of detergent, 0.8% Lubrol PX, t1/2: 20 days Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Bos taurus D-glucose + Glc2Man9GlcNAc2
-
?
Glc3Man9GlcNAc2 + H2O hydrolyses specifically terminal alpha1,2-linked glucose residue Bos taurus ?
-
?
Glc3Man9GlcNAc2 + H2O specific involvement in the N-linked oligosaccharide-processing pathway Bos taurus ?
-
?
Glc3Man9GlcNAc2 + H2O involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Bos taurus ?
-
?
Glc3Man9GlcNAc2 + H2O involved in processing of asparagine-linked oligosaccharides Bos taurus ?
-
?
additional information Glc1Man9GlcNAc2: not a substrate Bos taurus ?
-
?
additional information also acts, more slowly, on the corresponding glycolipids and glycopeptides Bos taurus ?
-
?
additional information p-nitrophenyl-alpha-D-glucopyranoside: not a substrate Bos taurus ?
-
?
additional information aryl-alpha-D-glucosides: not a substrate Bos taurus ?
-
?
additional information Glc2Man9GlcNAc2: not a substrate Bos taurus ?
-
?
additional information involved in first step of processing of oligosaccharides after transfer from dolichyl diphosphate to proteins Bos taurus ?
-
?
additional information involved in processing of asparagine-linked oligosaccharides Bos taurus ?
-
?
additional information involved in the formation of high mannose and complex glycoproteins Bos taurus ?
-
?

Subunits

Subunits Comment Organism
tetramer calf, 4 * 85000, SDS-PAGE Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
-
Bos taurus