Literature summary for 3.2.1.105 extracted from

Sun, J.; Peebles, C.A.
Engineering overexpression of ORCA3 and strictosidine glucosidase in Catharanthus roseus hairy roots increases alkaloid production (2016), Protoplasma, 253, 1255-1264 .

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Application

Application	Comment	Organism
pharmacology	Catharanthus roseus produces many pharmaceutically important terpenoid indole alkaloids (TIAs) such as vinblastine, vincristine, ajmalicine, and serpentine. Transcriptional regulator ORCA3 and strictosidine glucosidase (SGD) are both engineered in hairy roots under the control of a glucocorticoid-inducible promoter. Co-overexpression of ORCA3 and SGD result in a significant increase in serpentine by 44%, ajmalicine by 32%, catharanthine by 38%, tabersonine by 40%, lochnericine by 60% and hörhammericine by 56% . The total alkaloid pool is increased significantly by 47%. Combining overexpression of a positive regulator and a pathway gene which is not controlled by this regulator provides a way to enhance alkaloid production	Catharanthus roseus

Organism

Organism	UniProt	Comment	Textmining
Catharanthus roseus	Q9M7N7	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
hairy root	-	Catharanthus roseus	-

Synonyms

Synonyms	Comment	Organism
ORCA3	-	Catharanthus roseus

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Release 2023.1 (January 2023)