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Literature summary for 3.2.1.105 extracted from
- Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids (2002), Eur. J. Biochem., 269, 2204-2213.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| fusion protein with intein tag, expressed in Escherichia coli | Rauvolfia serpentina |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 8.8% inhibition at 1 mM | Rauvolfia serpentina |  |
| serpentine | 25.2% inhibition at 1 mM | Rauvolfia serpentina | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
strictosidine | pH 5, 30°C | Rauvolfia serpentina | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 450000 | - |
gel filtration | Rauvolfia serpentina |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rauvolfia serpentina | Q8GU20 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| homogeneity | Rauvolfia serpentina |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 18,19-dihydrostrictosidine + H2O | - |
Rauvolfia serpentina | D-glucose + 18,19-dihydrostrictosidine aglycone | - |
? | |
| 5alpha-carboxystrictosidine + H2O | - |
Rauvolfia serpentina | D-glucose + 5alpha-carboxystrictosidine aglycone | - |
? | |
| dolichantoside + H2O | - |
Rauvolfia serpentina | D-glucose + dolichantoside aglycone | - |
? | |
| additional information | no substrates: strictosidine pentaacetate, vincoside, strictosidine lactam, vincoside lactam, raucafficrine, 1,2-dihydrocafficrine, 1-methyl-1,2-dihydrocarcafficrine, secologanin, loganin, 4-nitrophenylglucoside, arbutin, vanillin-glucoside, isatinoxim-glucoside, prunasin, rhapontin, rutin, sinigrin and zeatin-glucoside | Rauvolfia serpentina | ? | - |
? | |
| Nbeta-acetylstrictosidine + H2O | weak substrate | Rauvolfia serpentina | D-glucose + Nbeta-acetylstrictosidine aglycone | - |
? | |
| Nbeta-methylstrictosidine + H2O | weak substrate | Rauvolfia serpentina | D-glucose + Nbeta-methylstrictosidine aglycone | - |
? | |
| strictosidine + H2O | - |
Rauvolfia serpentina | D-glucose + strictosidine aglycone | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Rauvolfia serpentina |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | - |
- |
Rauvolfia serpentina |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.2 | 8 | 50% of maximal activity at oH 4.2, above pH 5.2 the activity slowly decreases up to pH 8 | Rauvolfia serpentina |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Rauvolfia serpentina | calculated from amino acid sequence | - |
6 |
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Release 2023.1 (January 2023)
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