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Literature summary for 3.2.1.105 extracted from

  • Brandt, V.; Geerlings, A.; Tits, M.; Delaude, C.; van der Heijden, R.; Verpoorte, R.; Angenot, L.
    New strictosidine beta-glucosidase from Strychnos mellodora (2000), Plant Physiol. Biochem., 38, 187-192.
No PubMed abstract available
Search for more literature for 3.2.1.105

Inhibitors

Inhibitors Comment Organism Structure
D-gluconolactone strong inhibitor Catharanthus roseus
D-gluconolactone strong inhibitor Strychnos mellodora

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01 0.018 strictosidine
-
 Catharanthus roseus
0.12
-
 strictosidine
-
 Strychnos mellodora
0.12
-
 strictosidine pH 6.3, 30°C Strychnos mellodora
0.335
-
 palicoside
-
 Strychnos mellodora
0.335
-
 palicoside pH 6.3, 30°C Strychnos mellodora
0.564
-
 dolichantoside
-
 Strychnos mellodora
0.564
-
 dolichantoside pH 6.3, 30°C Strychnos mellodora
1.13
-
 dolichantoside
-
 Catharanthus roseus
1.13
-
 dolichantoside pH 6.3, 30°C Catharanthus roseus
7.015
-
 palicoside
-
 Catharanthus roseus
7.015
-
 palicoside pH 6.3, 30°C Catharanthus roseus
10
-
 strictosidine pH 6.3, 30°C Catharanthus roseus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 2 * 80000, SDS-PAGE Strychnos mellodora
80000
-
 x * 80000, SDS-PAGE Strychnos mellodora
200000
-
 native PAGE Strychnos mellodora
200000
-
 non-denaturing PAGE Strychnos mellodora

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Catharanthus roseus involved In the indole alkaloid biosynthesis by hydrolyzing strictosidine to cathenamine ?
-
 ?

Organism

Organism UniProt Comment Textmining
Catharanthus roseus
-
-
-
Strychnos mellodora
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Catharanthus roseus
-
 Strychnos mellodora

Source Tissue

Source Tissue Comment Organism Textmining
bark
-
 Strychnos mellodora
-
bark stem Strychnos mellodora
-
leaf
-
 Strychnos mellodora
-
root
-
 Strychnos mellodora
-
root bark Strychnos mellodora
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dolichantoside + H2O
-
 Strychnos mellodora ?
-
 ?
dolichantoside + H2O
-
 Catharanthus roseus D-glucose + dolichantoside aglycone
-
 ?
dolichantoside + H2O
-
 Strychnos mellodora D-glucose + dolichantoside aglycone
-
 ?
additional information involved In the indole alkaloid biosynthesis by hydrolyzing strictosidine to cathenamine Catharanthus roseus ?
-
 ?
palicoside + H2O
-
 Catharanthus roseus ?
-
 ?
palicoside + H2O
-
 Strychnos mellodora ?
-
 ?
palicoside + H2O
-
 Catharanthus roseus D-glucose + palicoside aglycone
-
 ?
palicoside + H2O
-
 Strychnos mellodora D-glucose + palicoside aglycone
-
 ?
strictosidine + H2O
-
 Strychnos mellodora D-glucose + strictosidine aglycone
-
 ?

Subunits

Subunits Comment Organism
?
-
 Catharanthus roseus
? x * 80000, SDS-PAGE Strychnos mellodora
dimer 2 * 80000, SDS-PAGE Strychnos mellodora

Synonyms

Synonyms Comment Organism
SGD
-
 Catharanthus roseus
SGD
-
 Strychnos mellodora