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Literature summary for 3.2.1.105 extracted from
- Purification and characterization of strictosidine beta-D-glucosidase from Catharanthus roseus cell suspension cultures (1998), Plant Physiol. Biochem., 36, 419-425.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 1 mM | Catharanthus roseus |  |
| serpentine | 1 mM | Catharanthus roseus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.018 | - |
strictosidine | - |
Catharanthus roseus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 63000 | - |
x * 63000, SDS-PAGE | Catharanthus roseus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| strictosidine | Catharanthus roseus | the enzyme may play an important role in steering the monoterpenoid indole alkaloid biosynthesis in a specific direction | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Catharanthus roseus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 3 different enzyme forms | Catharanthus roseus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | - |
Catharanthus roseus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Catharanthus roseus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, 50% loss of activity | Catharanthus roseus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| strictosidine | the enzyme may play an important role in steering the monoterpenoid indole alkaloid biosynthesis in a specific direction | Catharanthus roseus | ? | - |
? | |
| strictosidine + H2O | - |
Catharanthus roseus | D-glucose + strictosidine aglycone | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 63000, SDS-PAGE | Catharanthus roseus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
stable at | Catharanthus roseus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | - |
Catharanthus roseus |
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