Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 45% stimulation at 0.1 mM, hydrolysis of cholesteryl beta-D-glucoside. No effect on hydrolysis of p-nitrophenyl beta-D-glucoside | Sinapis alba | |
ethanol | 64% stimulation at a concentration of 20%, hydrolysis of cholesteryl beta-D-glucoside. 65% stimulation at a concentration of 20%, hydrolysis of p-nitrophenyl beta-D-glucoside | Sinapis alba |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5alpha-cholestanol | hydrolysis of cholesteryl glucoside | Sinapis alba | |
campesterol | hydrolysis of cholesteryl glucoside | Sinapis alba | |
cholesterol | hydrolysis of cholesteryl glucoside | Sinapis alba | |
ethanol | 39% inhibition at a concentration of 50%, hydrolysis of p-nitrophenyl beta-D-glucoside | Sinapis alba | |
gluconolactone | 50% inhibition at 4 mM, hydrolysis of cholesteryl beta-D-glucoside. 50% inhibition at 2 mM, hydrolysis of p-nitrophenyl beta-D-glucoside | Sinapis alba | |
sitosterol | hydrolysis of cholesteryl glucoside | Sinapis alba |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell membrane | - |
Sinapis alba | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
gel filtration | Sinapis alba |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sinapis alba | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Sinapis alba |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Sinapis alba | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.3621 | - |
- |
Sinapis alba |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesteryl beta-D-glucoside + H2O | - |
Sinapis alba | D-glucose + cholesterol | - |
? | |
p-nitrophenyl beta-D-glucoside + H2O | - |
Sinapis alba | D-glucose + p-nitrophenol | - |
? | |
sitosteryl beta-D-glucoside + H2O | - |
Sinapis alba | D-glucose + sitosterol | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
hydrolysis of cholesteryl beta-D-glucoside | Sinapis alba |
60 | - |
hydrolysis of p-nitrophenyl beta-D-glucoside | Sinapis alba |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.2 | 5.6 | hydrolysis of cholesteryl beta-D-glucoside or p-nitrophenyl beta-D-glucoside | Sinapis alba |