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Literature summary for 3.2.1.103 extracted from

  • Li, Y.T.; Nakagawa, H.; Kitamikado, M.; Li, S.C.
    endo-beta-Galactosidase from Escherichia freundii (1982), Methods Enzymol., 83, 610-619.
    View publication on PubMed

Application

Application Comment Organism
analysis useful for studying the structure of glycoproteins and oligosaccharides Citrobacter freundii

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, complete inhibition Citrobacter freundii
Cu2+ 1 mM, 30% inhibition Citrobacter freundii
Hg2+ 1 mM, complete inhibition Citrobacter freundii
PCMB 0.25 mM, complete inhibition Citrobacter freundii
Zn2+ 1 mM, 30% inhibition Citrobacter freundii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
gel filtration Citrobacter freundii

Organism

Organism UniProt Comment Textmining
Citrobacter freundii
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
Citrobacter freundii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Citrobacter freundii

Storage Stability

Storage Stability Organism
0°C, stable for several months Citrobacter freundii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Fucalpha1-2Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc-Cer + H2O
-
Citrobacter freundii Fucalpha1-2Galbeta1-3GlcNAcbeta1-3Gal + Glc-Cer
-
?
Galalpha1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc-Cer + H2O
-
Citrobacter freundii ?
-
?
Galbeta1-3[Fucalpha1-4]GlcNAcbeta1-3Galbeta1-4Glc-Cer + H2O
-
Citrobacter freundii Galbeta1-3[Fucalpha1-4]GlcNAcbeta1-3Gal + Glc-Cer
-
?
Galbeta1-4GlcNAcbeta1-3Galbeta1-3Galbeta1-4Glc-Cer + H2O
-
Citrobacter freundii Galbeta1-4GlcNAcbeta1-3Galbeta1-3Gal + Glc-Cer
-
?
polyglycosylceramides + H2O in absence of sodium taurodeoxycholate conversion into at least three shorter-chain glycosphingolipids, in presence of sodium taurodeoxycholate conversion into glucosylceramide Citrobacter freundii ? in absence of sodium taurodeoxycholate conversion into at least three shorter-chain glycosphingolipids, in presence of sodium taurodeoxycholate conversion into glucosylceramide ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
1 h, 50% loss of activity, bovine serum albumin, 0.4 mg/ml, prevents inactivation Citrobacter freundii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 5.8
-
Citrobacter freundii

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
17 h at room temperature, rapid loss of activity below Citrobacter freundii
4.5 5.5
-
Citrobacter freundii
6
-
-
Citrobacter freundii