Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Streptococcus pneumoniae |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 17% (v/v) polyethylene glycol 3350, 0.2 M ammonium sulfate, and 0.1 M sodium acetate trihydrate, pH 4.8 | Streptococcus pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.44 | - |
blood group A glycoconjugate | at 37°C | Streptococcus pneumoniae | |
0.57 | - |
blood group B glycoconjugate | at 37°C | Streptococcus pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | - |
- |
- |
Streptococcus pneumoniae SP3-BS71 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Sephacryl S-200 gel filtration | Streptococcus pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
blood group A glycoconjugate + H2O | Sp3GH98 is specific for the Galbeta1-4GlcNAc (type 2 core chain) linkage over either the Galbeta1-3GlcNAc (type 1 core chain) linkage or the Galbeta1-3GalNAc (type 4 core chain) and liberates the terminal GalNAcalpha1-3(Fucalpha1-2)Gal trisaccharide of the A antigen | Streptococcus pneumoniae | ? | - |
? | |
blood group A glycoconjugate + H2O | Sp3GH98 is specific for the Galbeta1-4GlcNAc (type 2 core chain) linkage over either the Galbeta1-3GlcNAc (type 1 core chain) linkage or the Galbeta1-3GalNAc (type 4 core chain) and liberates the terminal GalNAcalpha1-3(Fucalpha1-2)Gal trisaccharide of the A antigen | Streptococcus pneumoniae SP3-BS71 | ? | - |
? | |
blood group B glycoconjugate + H2O | Sp3GH98 is specific for the Galbeta1-4GlcNAc (type 2 core chain) linkage over either the Galbeta1-3GlcNAc (type 1 core chain) linkage or the Galbeta1-3GalNAc (type 4 core chain) and liberates the terminal Galalpha1-3(Fucalpha1-22)Gal trisaccharide of the B antigen | Streptococcus pneumoniae | ? | - |
? | |
blood group B glycoconjugate + H2O | Sp3GH98 is specific for the Galbeta1-4GlcNAc (type 2 core chain) linkage over either the Galbeta1-3GlcNAc (type 1 core chain) linkage or the Galbeta1-3GalNAc (type 4 core chain) and liberates the terminal Galalpha1-3(Fucalpha1-22)Gal trisaccharide of the B antigen | Streptococcus pneumoniae SP3-BS71 | ? | - |
? | |
LewisY tetrasaccharide + H2O | Sp4GH98 is able to hydrolyze the Galbeta1-4GalNAc linkage within the LewisY antigen | Streptococcus pneumoniae | ? | - |
? | |
LewisY tetrasaccharide + H2O | Sp4GH98 is able to hydrolyze the Galbeta1-4GalNAc linkage within the LewisY antigen | Streptococcus pneumoniae SP3-BS71 | ? | - |
? | |
additional information | Sp3GH98 does not cleave the LewisY tetrasaccharide | Streptococcus pneumoniae | ? | - |
? | |
additional information | Sp3GH98 does not cleave the LewisY tetrasaccharide | Streptococcus pneumoniae SP3-BS71 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endo-beta-galactosidase | - |
Streptococcus pneumoniae |
Sp3GH98 | - |
Streptococcus pneumoniae |
Sp4GH98 | - |
Streptococcus pneumoniae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.67 | - |
blood group A glycoconjugate | at 37°C | Streptococcus pneumoniae | |
11.17 | - |
blood group B glycoconjugate | at 37°C | Streptococcus pneumoniae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
LewisY tetrasaccharide | at 37°C | Streptococcus pneumoniae | |
10.67 | - |
blood group A glycoconjugate | at 37°C | Streptococcus pneumoniae | |
20 | - |
blood group B glycoconjugate | at 37°C | Streptococcus pneumoniae |