Literature summary for 3.2.1.102 extracted from

Anderson, K.M.; Ashida, H.; Maskos, K.; Dell, A.; Li, S.C.; Li, Y.T.
A clostridial endo-beta-galactosidase that cleaves both blood group A and B glycotopes: the first member of a new glycoside hydrolase family, GH98 (2005), J. Biol. Chem., 280, 7720-7728.

Search for more literature for 3.2.1.102

Cloned(Commentary)

Cloned (Comment)	Organism
gene eabC, DNA and amino acid sequence determination and analysis, functional overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3)	Clostridium perfringens

Organism

Organism	UniProt	Comment	Textmining
Clostridium perfringens	Q6RUF5	strain 13, gene eabC	-
Clostridium perfringens ATCC 10543	Q6RUF5	strain 13, gene eabC	-

Purification (Commentary)

Purification (Comment)	Organism
native enzyme from strain ATCC 10543, by gel filtration, ion exchange, adsorption, and concanavalin A affinity chromatography, and another step of ion exchange chromatography, 2630fold to homogeneity, recombinant His-tagged enzyme by nickel affinity chromatography	Clostridium perfringens

Source Tissue

Source Tissue	Comment	Organism	Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2894	-	purified native enzyme	Clostridium perfringens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
blood group A glycoconjugate + H2O	substrate from human erythrocytes and from porcine gastric mucin, liberation of the A trisaccharide GalNAcalpha(1-3)fucosealpha(1-2)galactose, product identification by NMR	Clostridium perfringens	?	-	?
blood group A glycoconjugate + H2O	substrate from human erythrocytes and from porcine gastric mucin, liberation of the A trisaccharide GalNAcalpha(1-3)fucosealpha(1-2)galactose, product identification by NMR	Clostridium perfringens ATCC 10543	?	-	?
blood group B glycoconjugate + H2O	substrate from human erythrocytes and human ovarian cyst, liberation of the B trisaccharide Galalpha(1-3)fucosealpha(1-2)galactose, product identification by NMR	Clostridium perfringens	?	-	?
blood group B glycoconjugate + H2O	substrate from human erythrocytes and human ovarian cyst, liberation of the B trisaccharide Galalpha(1-3)fucosealpha(1-2)galactose, product identification by NMR	Clostridium perfringens ATCC 10543	?	-	?

Synonyms

Synonyms	Comment	Organism
blood group A- and B-cleaving endo-beta-galactosidase	-	Clostridium perfringens
E-ABase	-	Clostridium perfringens
More	the enzyme belongs to the GH98 family	Clostridium perfringens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Clostridium perfringens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.5	-	assay at	Clostridium perfringens

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Release 2023.1 (January 2023)