Cloned (Comment) | Organism |
---|---|
expressed in Sulfolobus acidocaldarius. Attempts to obtain soluble enzyme from Escherichia coli strains are unsuccessful | Sulfolobus acidocaldarius |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Sulfolobus acidocaldarius | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
12 * 72000, SDS-PAGE | Sulfolobus acidocaldarius |
73000 | - |
12 * 73000, calculated from sequence | Sulfolobus acidocaldarius |
914000 | - |
gel filtration | Sulfolobus acidocaldarius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfolobus acidocaldarius | Q4J9M3 | - |
- |
Sulfolobus acidocaldarius DSM 639 | Q4J9M3 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Sulfolobus acidocaldarius |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:maltose-grown cell | the transcription level of malA is increased 3fold upon the addition of maltose or starch to the medium. The alpha-glucosidase activity for maltose as a substrate in cell extracts is 11fold higher during growth in YT medium (Brocks mineral salts, 0.1% (w/v) tryptone, and 0.005% (w/v) yeast extract) containing maltose, than during growth on other sugars | Sulfolobus acidocaldarius | - |
culture condition:starch-grown cell | the transcription level of malA is increased 3fold upon the addition of maltose or starch to the medium. The alpha-glucosidase activity for starch as a substrate in cell extracts is 10fold higher during growth in YT medium (Brocks mineral salts, 0.1% (w/v) tryptone, and 0.005% (w/v) yeast extract) containing starch, than during growth on other sugars | Sulfolobus acidocaldarius | - |
Storage Stability | Organism |
---|---|
4°C, stored for at least 6 months without any loss of activity | Sulfolobus acidocaldarius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isomaltose + H2O | i.e. alpha-D-Glc-(1->6)-D-Glc. Multifunctional enzyme that catalyzes also terminal alpha-1,4-linked, alpha-1,2-linked and alpha-1,3-linked glucose residues (EC 3.2.1.20/kojibiose/EC 3.2.1.84 hydrolase) | Sulfolobus acidocaldarius | alpha-D-glucose + D-glucose | - |
? | |
isomaltose + H2O | i.e. alpha-D-Glc-(1->6)-D-Glc. Multifunctional enzyme that catalyzes also terminal alpha-1,4-linked, alpha-1,2-linked and alpha-1,3-linked glucose residues (EC 3.2.1.20/kojibiose/EC 3.2.1.84 hydrolase) | Sulfolobus acidocaldarius DSM 639 | alpha-D-glucose + D-glucose | - |
? | |
panose + H2O | alpha-D-Glc-(1->6)-alpha-D-Glc-(1->4)-D-Glc. Multifunctional enzyme that catalyzes also terminal alpha-1,4-linked, alpha-1,2-linked and alpha-1,3-linked glucose residues (EC 3.2.1.20/kojibiose/EC 3.2.1.84 hydrolase) | Sulfolobus acidocaldarius | maltose + alpha-D-glucose | - |
? | |
panose + H2O | alpha-D-Glc-(1->6)-alpha-D-Glc-(1->4)-D-Glc. Multifunctional enzyme that catalyzes also terminal alpha-1,4-linked, alpha-1,2-linked and alpha-1,3-linked glucose residues (EC 3.2.1.20/kojibiose/EC 3.2.1.84 hydrolase) | Sulfolobus acidocaldarius DSM 639 | maltose + alpha-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dodecamer | 12 * 72000, SDS-PAGE | Sulfolobus acidocaldarius |
dodecamer | 12 * 73000, calculated from sequence | Sulfolobus acidocaldarius |
Synonyms | Comment | Organism |
---|---|---|
MalA | - |
Sulfolobus acidocaldarius |
Saci1160 | locus name | Sulfolobus acidocaldarius |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
half-life: 33.8 h | Sulfolobus acidocaldarius |
100 | - |
half-life: 10.6 h | Sulfolobus acidocaldarius |
105 | - |
half-life: 1.8 h | Sulfolobus acidocaldarius |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Sulfolobus acidocaldarius | calculated from sequence | - |
5.9 |
Organism | Comment | Expression |
---|---|---|
Sulfolobus acidocaldarius | the transcription level of malA is increased 3fold upon the addition of maltose or starch to the medium | up |