Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.10 extracted from

  • Noguchi, A.; Yano, M.; Ohshima, Y.; Hemmi, H.; Inohara-ochiai, M.; Okada, M.; Min, K.s.; Nakayama, T.; Nishino, T.
    Deciphering the molecular basis of the broad substrate specificity of alpha-glucosidase from Bacillus sp. SAM1606 (2003), J. Biochem., 134, 543-550.
    View publication on PubMed
Search for more literature for 3.2.1.10

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Escherichia coli strain W3110 Bacillus sp. (in: Bacteria)

Protein Variants

Protein Variants Comment Organism
N342T site-directed mutagenesis, reduced activity with isomaltose, altered substrate specificity Bacillus sp. (in: Bacteria)
P273G site-directed mutagenesis, increased activity with trehalose, reduced activity with isomaltose, altered substrate specificity Bacillus sp. (in: Bacteria)
P273G/N342T site-directed mutagenesis, reduced activity with isomaltose, altered substrate specificity Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
 trehalose mutant P273G/N342T, pH 7.2, 35°C Bacillus sp. (in: Bacteria)
5.3
-
 isomaltose wild-type enzyme, pH 7.2, 35°C Bacillus sp. (in: Bacteria)
13
-
 isomaltose mutant N342T, pH 7.2, 35°C Bacillus sp. (in: Bacteria)
14
-
 isomaltose mutant P273G, pH 7.2, 35°C Bacillus sp. (in: Bacteria)
14
-
 trehalose mutant P273G, pH 7.2, 35°C Bacillus sp. (in: Bacteria)
20
-
 isomaltose mutant P273G/N342T, pH 7.2, 35°C Bacillus sp. (in: Bacteria)
320
-
 trehalose wild-type enzyme, pH 7.2, 35°C Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bacillus sp. (in: Bacteria) evolutionary connection to other alpha-glucosidases in comparison of amino acid sequence and substrate specificity, overview, the alpha-glucosidase, EC 3.2.1.20, of Bacillus sp. strain SAM1606 shows 192fold enhanced activity with trehalose by mutations P273G and T342N ?
-
 ?
additional information Bacillus sp. (in: Bacteria) SAM1606 evolutionary connection to other alpha-glucosidases in comparison of amino acid sequence and substrate specificity, overview, the alpha-glucosidase, EC 3.2.1.20, of Bacillus sp. strain SAM1606 shows 192fold enhanced activity with trehalose by mutations P273G and T342N ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) SAM1606
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl 1-O-alpha-D-glucopyranoside + H2O
-
 Bacillus sp. (in: Bacteria) 4-nitrophenol + alpha-D-glucopyranose
-
 ?
4-nitrophenyl 1-O-alpha-D-glucopyranoside + H2O
-
 Bacillus sp. (in: Bacteria) SAM1606 4-nitrophenol + alpha-D-glucopyranose
-
 ?
isomaltose + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
isomaltose + H2O
-
 Bacillus sp. (in: Bacteria) SAM1606 ?
-
 ?
additional information evolutionary connection to other alpha-glucosidases in comparison of amino acid sequence and substrate specificity, overview, the alpha-glucosidase, EC 3.2.1.20, of Bacillus sp. strain SAM1606 shows 192fold enhanced activity with trehalose by mutations P273G and T342N Bacillus sp. (in: Bacteria) ?
-
 ?
additional information enzyme is highly specific for alpha-1,6-linkages Bacillus sp. (in: Bacteria) ?
-
 ?
additional information evolutionary connection to other alpha-glucosidases in comparison of amino acid sequence and substrate specificity, overview, the alpha-glucosidase, EC 3.2.1.20, of Bacillus sp. strain SAM1606 shows 192fold enhanced activity with trehalose by mutations P273G and T342N Bacillus sp. (in: Bacteria) SAM1606 ?
-
 ?
additional information enzyme is highly specific for alpha-1,6-linkages Bacillus sp. (in: Bacteria) SAM1606 ?
-
 ?
trehalose + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
trehalose + H2O
-
 Bacillus sp. (in: Bacteria) SAM1606 ?
-
 ?

Synonyms

Synonyms Comment Organism
dextrin 6-alpha-glucanohydrolase
-
 Bacillus sp. (in: Bacteria)
More enzyme belongs to the family 13 of glycosyl hydrolases Bacillus sp. (in: Bacteria)
O16G
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 assay at Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Bacillus sp. (in: Bacteria)