Cloned (Comment) | Organism |
---|---|
overexpression of wild-type and mutant enzymes in Escherichia coli MV1184 | Bacillus cereus |
Protein Variants | Comment | Organism |
---|---|---|
D199N | site-directed mutagenesis, active site mutant, highly reduced activity compared to the wild-type enzyme | Bacillus cereus |
D329N | site-directed mutagenesis, active site mutant, catalytically inactive mutant | Bacillus cereus |
E255Q | site-directed mutagenesis, active site mutant, highly reduced activity compared to the wild-type enzyme | Bacillus cereus |
H103N | site-directed mutagenesis, substrate binding mutant, about 50% reduced activity compared to the wild-type enzyme | Bacillus cereus |
H328N | site-directed mutagenesis, substrate binding mutant, highly reduced activity compared to the wild-type enzyme | Bacillus cereus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
4-nitrophenyl alpha-D-glucopyranoside | recombinant mutant D199N, pH 7.0, 35°C | Bacillus cereus | |
0.015 | - |
4-nitrophenyl alpha-D-glucopyranoside | recombinant mutant E255Q, pH 7.0, 35°C | Bacillus cereus | |
0.8 | - |
4-nitrophenyl alpha-D-glucopyranoside | recombinant wild-type enzyme, pH 7.0, 35°C | Bacillus cereus | |
0.99 | - |
4-nitrophenyl alpha-D-glucopyranoside | recombinant mutant H103N, pH 7.0, 35°C | Bacillus cereus | |
13.3 | - |
4-nitrophenyl alpha-D-glucopyranoside | recombinant mutant H328N, pH 7.0, 35°C | Bacillus cereus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
strain ATCC 7064 | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli MV1184 | Bacillus cereus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
alpha-D-glucopyranosyl-(1-4)-[alpha-D-glucopyranosyl-(1-6)]-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranose + H2O = alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranose + D-glucose | active sites residues are Asp199, Glu255, and Asp329, substrate binding residues are His103, and His328, catalytic mechanism from comparison with other enzymes' primary and crystal enzyme structures | Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Bacillus cereus | 4-nitrophenol + alpha-D-glucopyranose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dextrin 6-alpha-D-glucanohydrolase | - |
Bacillus cereus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Bacillus cereus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
4-nitrophenyl alpha-D-glucopyranoside | recombinant mutant D199N, pH 7.0, 35°C | Bacillus cereus | |
0.39 | - |
4-nitrophenyl alpha-D-glucopyranoside | recombinant mutant E255Q, pH 7.0, 35°C | Bacillus cereus | |
3.11 | - |
4-nitrophenyl alpha-D-glucopyranoside | recombinant mutant H328N, pH 7.0, 35°C | Bacillus cereus | |
352 | - |
4-nitrophenyl alpha-D-glucopyranoside | recombinant mutant H103N, pH 7.0, 35°C | Bacillus cereus | |
483 | - |
4-nitrophenyl alpha-D-glucopyranoside | recombinant wild-type enzyme, pH 7.0, 35°C | Bacillus cereus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Bacillus cereus |