Cloned (Comment) | Organism |
---|---|
construction of genetic library, DNA sequence determination and analysis of gene aglA, amino acid sequence determination, expression of the truncated enzyme fused to the N-terminus of lacZ in Escherichia coli XL1-Blue | Bifidobacterium adolescentis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.05 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 6.5, 30°C | Bifidobacterium adolescentis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
gel filtration | Bifidobacterium adolescentis |
71000 | - |
1 * 71000, SDS-PAGE, 1 * 72138, recombinant fusion protein, amino acid sequence calculation | Bifidobacterium adolescentis |
72138 | - |
1 * 71000, SDS-PAGE, 1 * 72138, recombinant fusion protein, amino acid sequence calculation | Bifidobacterium adolescentis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bifidobacterium adolescentis | Q9AF93 | strain DSM 20083, gene aglA | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli | Bifidobacterium adolescentis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl alpha-D-glucoside + H2O | - |
Bifidobacterium adolescentis | 4-methylumbelliferol + alpha-D-glucose | - |
? | |
4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Bifidobacterium adolescentis | 4-nitrophenol + alpha-D-glucopyranose | - |
? | |
isomaltose + H2O | - |
Bifidobacterium adolescentis | ? | - |
? | |
isomaltriose + H2O | - |
Bifidobacterium adolescentis | ? | - |
? | |
additional information | substrate specificity, enzyme is also capable to perform a transglycosylation producing oligosaccharides from trehalose and with low activity from sucrose, but no hydrolytic activity is detected with sucrose | Bifidobacterium adolescentis | ? | - |
? | |
trehalose + H2O | low activity | Bifidobacterium adolescentis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 71000, SDS-PAGE, 1 * 72138, recombinant fusion protein, amino acid sequence calculation | Bifidobacterium adolescentis |
Synonyms | Comment | Organism |
---|---|---|
AglA | - |
Bifidobacterium adolescentis |
More | the enzyme belongs to family 13 of glycosyl gydrolases | Bifidobacterium adolescentis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Bifidobacterium adolescentis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | - |
- |
Bifidobacterium adolescentis |