Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme lacking the native signal peptide in Escherichia coli strain BL21(DE3) | Pseudoalteromonas sp. 2-3 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | with substrate potato starch the enzyme shows a Km of 6.94 mg/ml and Vmax of 0.27 mg/ml * min | Pseudoalteromonas sp. 2-3 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | strongly activating | Pseudoalteromonas sp. 2-3 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudoalteromonas sp. 2-3 | W8SKF6 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme lacking the native signal peptide 7fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Pseudoalteromonas sp. 2-3 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
36.7 | - |
purified enzyme without Ca2+, pH 8.0, 20°C | Pseudoalteromonas sp. 2-3 |
141.5 | - |
purified enzyme with 5 mM Ca2+, pH 8.0, 20°C | Pseudoalteromonas sp. 2-3 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | - |
Pseudoalteromonas sp. 2-3 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 68800, recombinant enzyme, SDS-PAGE | Pseudoalteromonas sp. 2-3 |
Synonyms | Comment | Organism |
---|---|---|
cold-active alpha-amylase | - |
Pseudoalteromonas sp. 2-3 |
psychrophilic alpha amylase | UniProt | Pseudoalteromonas sp. 2-3 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
- |
Pseudoalteromonas sp. 2-3 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 30 | over 40% activity within this range, profile overview | Pseudoalteromonas sp. 2-3 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified recombinant His-tagged enzyme retaining 63% of its activity after 10 min incubation | Pseudoalteromonas sp. 2-3 |
43 | - |
purified recombinant His-tagged enzyme retaining 50% of its activity after 10 min incubation | Pseudoalteromonas sp. 2-3 |
50 | - |
purified recombinant His-tagged enzyme, inactivation after 10 min incubation | Pseudoalteromonas sp. 2-3 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudoalteromonas sp. 2-3 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | activity range, profile overview | Pseudoalteromonas sp. 2-3 |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the glycosyl hydrolase family 13, GH13 | Pseudoalteromonas sp. 2-3 |
additional information | the catalytic triad is composed of conserved residues Asp309, Glu338, and Asp417 | Pseudoalteromonas sp. 2-3 |