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Literature summary for 3.2.1.1 extracted from

  • Sanchez, A.C.; Ravanal, M.C.; Andrews, B.A.; Asenjo, J.A.
    Heterologous expression and biochemical characterization of a novel cold-active alpha-amylase from the Antarctic bacteria Pseudoalteromonas sp. 2-3 (2019), Protein Expr. Purif., 155, 78-85 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme lacking the native signal peptide in Escherichia coli strain BL21(DE3) Pseudoalteromonas sp. 2-3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information with substrate potato starch the enzyme shows a Km of 6.94 mg/ml and Vmax of 0.27 mg/ml * min Pseudoalteromonas sp. 2-3

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ strongly activating Pseudoalteromonas sp. 2-3

Organism

Organism UniProt Comment Textmining
Pseudoalteromonas sp. 2-3 W8SKF6
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme lacking the native signal peptide 7fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Pseudoalteromonas sp. 2-3

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
36.7
-
purified enzyme without Ca2+, pH 8.0, 20°C Pseudoalteromonas sp. 2-3
141.5
-
purified enzyme with 5 mM Ca2+, pH 8.0, 20°C Pseudoalteromonas sp. 2-3

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
starch + H2O
-
Pseudoalteromonas sp. 2-3 ?
-
?

Subunits

Subunits Comment Organism
? x * 68800, recombinant enzyme, SDS-PAGE Pseudoalteromonas sp. 2-3

Synonyms

Synonyms Comment Organism
cold-active alpha-amylase
-
Pseudoalteromonas sp. 2-3
psychrophilic alpha amylase UniProt Pseudoalteromonas sp. 2-3

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
-
Pseudoalteromonas sp. 2-3

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 30 over 40% activity within this range, profile overview Pseudoalteromonas sp. 2-3

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
purified recombinant His-tagged enzyme retaining 63% of its activity after 10 min incubation Pseudoalteromonas sp. 2-3
43
-
purified recombinant His-tagged enzyme retaining 50% of its activity after 10 min incubation Pseudoalteromonas sp. 2-3
50
-
purified recombinant His-tagged enzyme, inactivation after 10 min incubation Pseudoalteromonas sp. 2-3

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Pseudoalteromonas sp. 2-3

pH Range

pH Minimum pH Maximum Comment Organism
6 9 activity range, profile overview Pseudoalteromonas sp. 2-3

General Information

General Information Comment Organism
evolution the enzyme belongs to the glycosyl hydrolase family 13, GH13 Pseudoalteromonas sp. 2-3
additional information the catalytic triad is composed of conserved residues Asp309, Glu338, and Asp417 Pseudoalteromonas sp. 2-3