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Literature summary for 3.2.1.1 extracted from
- Characteristics and thermodynamics of alpha-amylase from thermophilic actinobacterium, Laceyella sacchari TSI-2 (2015), Process Biochem., 50, 2128-2136 .
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Thiourea | - |
Laceyella sacchari |  |
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | the enzyme is highly efficient in removing starch stains from the cotton cloth | Laceyella sacchari |
General Stability
| General Stability | Organism |
|---|---|
| thiourea stabilizes | Laceyella sacchari |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Laceyella sacchari | |
| Ca2+ | - |
Laceyella sacchari | |
| EDTA | - |
Laceyella sacchari | |
| Fe3+ | - |
Laceyella sacchari | |
| Hg2+ | complete inhibition | Laceyella sacchari | |
| K+ | - |
Laceyella sacchari | |
| Mn2+ | complete inhibition | Laceyella sacchari | |
| Na+ | - |
Laceyella sacchari | |
| Ni2+ | complete inhibition | Laceyella sacchari | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km is 2.71 mg/ml, pH 7.0, 50°C, kinetics and thermodynamics, overview | Laceyella sacchari |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
about, native PAGE | Laceyella sacchari |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Laceyella sacchari | - |
isolated from the nearby soil of a hot water spring (45-50°C) located in Tul-siShyam, Gujarat, India (latitude 21.0517, longitude 71.0256) | - |
| Laceyella sacchari TSI-2 | - |
isolated from the nearby soil of a hot water spring (45-50°C) located in Tul-siShyam, Gujarat, India (latitude 21.0517, longitude 71.0256) | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 55fold from culture supernatant by acetone precipitation, ultrafiltration, and gel filtration | Laceyella sacchari |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | strain TSI2 optimally produces alpha-amylase at 50°C and pH 7.0 on agar agar medium | Laceyella sacchari | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4215.91 | - |
purified enzyme, pH 7.0, 50°C | Laceyella sacchari |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme is highly efficient in removing starch stains from the cotton cloth | Laceyella sacchari | ? | - |
? | |
| additional information | the enzyme is highly efficient in removing starch stains from the cotton cloth | Laceyella sacchari TSI-2 | ? | - |
? | |
| starch + H2O | 1% starch solution, alpha-amylase is an endo-hydrolyase, which randomly acts within starch chains producing a mixture of maltooligosaccharides along with maltose and glucose | Laceyella sacchari | ? | - |
? | |
| starch + H2O | 1% starch solution, alpha-amylase is an endo-hydrolyase, which randomly acts within starch chains producing a mixture of maltooligosaccharides along with maltose and glucose | Laceyella sacchari TSI-2 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 31000, SDS-PAGE | Laceyella sacchari |
| More | the native enzyme contains 4.49% alpha-helices and 43.69% beta-sheets, while the denatured enzyme contains 11.53% alpha-helices and 28.35% beta-sheets | Laceyella sacchari |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Laceyella sacchari |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 100 | activity range | Laceyella sacchari |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
purified enzyme, half-life is 300 min | Laceyella sacchari |
| 60 | - |
purified enzyme, half-life is 720 min | Laceyella sacchari |
| 70 | - |
purified enzyme, half-life is 1080 min | Laceyella sacchari |
| 80 | - |
purified enzyme, half-life is 540 min | Laceyella sacchari |
| 90 | - |
purified enzyme, half-life is 300 min | Laceyella sacchari |
| 100 | - |
purified enzyme, half-life is 120 min | Laceyella sacchari |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Laceyella sacchari |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | ativity range | Laceyella sacchari |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | purified enzyme, 12 h, loss of 40% activity. The enzyme is relatively less stable at pH 6.0, pH 8.0 and pH 9.0 and is completely denatured at pH 4.0 and pH 5.0 | Laceyella sacchari |
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