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Literature summary for 3.2.1.1 extracted from
- Purification and characterisation of alpha-amylase produced by mutant strain of Aspergillus oryzae EMS-18 (2015), Nat. Prod. Res., 29, 710-716 .
No PubMed abstract available
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Aspergillus oryzae | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Aspergillus oryzae |  |
| Co2+ | activates | Aspergillus oryzae | |
| additional information | poor effects by Mg2+, Na+, Ni2+, Zn2+, Cu2+, Fe2+, and Ba2+ | Aspergillus oryzae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Aspergillus oryzae | the extracellular enzyme hydrolyses alpha-1,4 glucosidic linkages randomly throughout the starch molecule in an endo-fashion producing oligosaccharides and monosaccharides, including maltose, glucose and alpha limit dextrin | ? | - |
? | |
| additional information | Aspergillus oryzae EMS-18 | the extracellular enzyme hydrolyses alpha-1,4 glucosidic linkages randomly throughout the starch molecule in an endo-fashion producing oligosaccharides and monosaccharides, including maltose, glucose and alpha limit dextrin | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | - |
- |
- |
| Aspergillus oryzae EMS-18 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| extracellular enzyme from culture supernatant 9.5fold by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration | Aspergillus oryzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the extracellular enzyme hydrolyses alpha-1,4 glucosidic linkages randomly throughout the starch molecule in an endo-fashion producing oligosaccharides and monosaccharides, including maltose, glucose and alpha limit dextrin | Aspergillus oryzae | ? | - |
? | |
| additional information | the extracellular enzyme hydrolyses alpha-1,4 glucosidic linkages randomly throughout the starch molecule in an endo-fashion producing oligosaccharides and monosaccharides, including maltose, glucose and alpha limit dextrin | Aspergillus oryzae EMS-18 | ? | - |
? | |
| starch + H2O | - |
Aspergillus oryzae | ? | - |
? | |
| starch + H2O | - |
Aspergillus oryzae EMS-18 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 48000, SDS-PAGE | Aspergillus oryzae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-1,4 glucan-glucanohydrolase | - |
Aspergillus oryzae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
broad optimum | Aspergillus oryzae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 70 | over 80% of maximal activity within this range | Aspergillus oryzae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Aspergillus oryzae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7 | over 80% of maximal activity within this range | Aspergillus oryzae |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | enzyme activity is equally high in distilled water, citrate buffer, acetate buffer, and phosphate buffer | Aspergillus oryzae |
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Release 2023.1 (January 2023)
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