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Literature summary for 3.2.1.1 extracted from

  • Sudan, S.K.; Kumar, N.; Kaur, I.; Sahni, G.
    Production, purification and characterization of raw starch hydrolyzing thermostable acidic alpha-amylase from hot springs, India (2018), Int. J. Biol. Macromol., 117, 831-839 .
    View publication on PubMed
Search for more literature for 3.2.1.1

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 20% inhibition at 1 mM, 30% at 5 mM Geobacillus sp. K1C
Cu2+ complete inhibition at 1 mM Geobacillus sp. K1C
Dextran complete inhibition at 1% Geobacillus sp. K1C
DTT 20% inhibition at 1 mM Geobacillus sp. K1C
EDTA over 80% inhibition at 1 mM Geobacillus sp. K1C
ethanol 20% inhibition at 10%, 40% at 20% Geobacillus sp. K1C
glycerol 20-25% inhibition at 1-2% Geobacillus sp. K1C
glycine almost complete inhibition at 1% Geobacillus sp. K1C
Hg2+ complete inhibition at 1 mM Geobacillus sp. K1C
Isoamylalcohol 70% inhibition at 10%, 90% at 20% Geobacillus sp. K1C
methanol 20% inhibition at 10%, 40% at 20% Geobacillus sp. K1C
Mg2+ 30% inhibition at 1 mM, 50% at 5 mM Geobacillus sp. K1C
Mn2+ 80% inhibition at 1-5 mM Geobacillus sp. K1C
additional information no or poor effect by 10-20% of acetone and benzol Geobacillus sp. K1C
Ni2+ no inhibition at 1 mM, 50% at 5 mM Geobacillus sp. K1C
Pb2+ 90% inhibition at 1 mM Geobacillus sp. K1C
PEG 8000 15-20% inhibition at 1-2% Geobacillus sp. K1C
PMSF 10% inhibition at 1 mM, 90% at 5 mM Geobacillus sp. K1C
Propanol 30% inhibition at 10%, 50% at 20% Geobacillus sp. K1C
SDS 40% inhibition at 0.1-0.2% Geobacillus sp. K1C
Triton X-100 15% inhibition at 0.1%, 20% at 0.2% Geobacillus sp. K1C
Tween 20 20% inhibition at 0.1-0.2% Geobacillus sp. K1C
Tween 80 10% inhibition at 0.1%, 25% at 0.2% Geobacillus sp. K1C

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the activity of the purified enzyme is Ca2+-independent Geobacillus sp. K1C

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
 about, gel filtration Geobacillus sp. K1C

Organism

Organism UniProt Comment Textmining
Geobacillus sp. K1C
-
 isolated from Manikaran hot springs, India
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme 49fold by ammonium sulfate fractionation, dialysis, anion exchange chromatography, and gel filtration Geobacillus sp. K1C

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
26493
-
 purified native enzyme, pH 6.0, 80°C, substrate soluble starch Geobacillus sp. K1C

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity, overview Geobacillus sp. K1C ?
-
 ?
raw corn starch + H2O
-
 Geobacillus sp. K1C ?
-
 ?
raw potato starch + H2O
-
 Geobacillus sp. K1C ?
-
 ?
raw rice starch + H2O
-
 Geobacillus sp. K1C ?
-
 ?
raw wheat starch + H2O
-
 Geobacillus sp. K1C ?
-
 ?
soluble starch + H2O 0.5% starch solution Geobacillus sp. K1C ?
-
 ?

Subunits

Subunits Comment Organism
? x * 59000, SDS-PAGE Geobacillus sp. K1C
More presence of random coil conformation Geobacillus sp. K1C

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Geobacillus sp. K1C

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 95 over 50% of maximal activity within this range, profile overview Geobacillus sp. K1C

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 in the presence of substrate, thermostability of the alpha-amylase is increased Geobacillus sp. K1C
70
-
 purified native enzyme, 40% activity is retained after 2 h, 20% after 5 h in absence of substrate, while in presence of 0.1% starch substrate, over 80% activity is retained for 8 h Geobacillus sp. K1C
80
-
 purified native enzyme, 15% activity is retained after 1 h, 10% after 2 h in absence of substrate, while in presence of 0.1% starch substrate, about 30% activity is retained for 4-8 h, 20% remaining after 9 h Geobacillus sp. K1C

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Geobacillus sp. K1C

pH Range

pH Minimum pH Maximum Comment Organism
4 10 activity range, profile overview Geobacillus sp. K1C

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 7 purified native enzyme, 90% initial activity is retained after 48 h Geobacillus sp. K1C