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Literature summary for 3.2.1.1 extracted from
- Improvement of enzymatic properties of Rhizopus oryzae alpha-amylase by site-saturation mutagenesis of histidine 286 (2018), Enzyme Microb. Technol., 117, 96-102 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H286A | site-directed mutagenesis, altered activity compared to wild-type | Rhizopus arrhizus |
| H286C | site-directed mutagenesis, altered activity compared to wild-type | Rhizopus arrhizus |
| H286D | site-directed mutagenesis, the mutant shows a decreased optimum pH compared to wild-type, altered activity compared to wild-type | Rhizopus arrhizus |
| H286E | site-directed mutagenesis, the mutant shows a 6.43fold increase in half-life at pH 4.5 and an decreased pH optimum compared to wild-type, altered activity compared to wild-type | Rhizopus arrhizus |
| H286F | site-directed mutagenesis, altered activity compared to wild-type | Rhizopus arrhizus |
| H286G | site-directed mutagenesis, altered activity compared to wild-type | Rhizopus arrhizus |
| H286I | site-directed mutagenesis, mutant H286I shows a 1.5fold increase in half-life at 55°C compared to wild-type, and the mutant shows an increased optimum temperature and a decreased pH optimum compared to wild-type, altered activity compared to wild-type | Rhizopus arrhizus |
| H286K | site-directed mutagenesis, altered activity compared to wild-type | Rhizopus arrhizus |
| H286L | site-directed mutagenesis, the mutant shows an increased optimum temperature and a decreased pH optimum compared to wild-type, the ability to form maltose from soluble starch is significantly improved, altered activity compared to wild-type | Rhizopus arrhizus |
| H286M | site-directed mutagenesis, the mutant shows an inproved ability to form maltose from soluble starch compared to wild-type, altered activity compared to wild-type | Rhizopus arrhizus |
| H286N | site-directed mutagenesis, altered activity compared to wild-type | Rhizopus arrhizus |
| H286P | site-directed mutagenesis, the mutant shows an increased optimum temperature compared to wild-type, altered activity compared to wild-type | Rhizopus arrhizus |
| H286Q | site-directed mutagenesis, altered activity compared to wild-type | Rhizopus arrhizus |
| H286R | site-directed mutagenesis, altered activity compared to wild-type | Rhizopus arrhizus |
| H286S | site-directed mutagenesis, the mutant shows an increased optimum temperature compared to wild-type, altered activity compared to wild-type | Rhizopus arrhizus |
| H286T | site-directed mutagenesis, the mutant shows an increased optimum temperature compared to wild-type, altered activity compared to wild-type | Rhizopus arrhizus |
| H286V | site-directed mutagenesis, altered activity compared to wild-type | Rhizopus arrhizus |
| H286W | site-directed mutagenesis, altered activity compared to wild-type | Rhizopus arrhizus |
| H286Y | site-directed mutagenesis, altered activity compared to wild-type | Rhizopus arrhizus |
| additional information | activities and half-lives of mutant enzymes compared to wild-type enzyme at different pH values, and molecular docking of maltotriose to wild-type and mutant enzymes, overview | Rhizopus arrhizus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics of wild-type and mutant enzymes, overview | Rhizopus arrhizus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | Rhizopus arrhizus | - |
D-glucose + maltose + maltotriose | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhizopus arrhizus | A0A1P8L1E1 | i.e. Rhizopus arrhizus var. delemar | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | - |
Rhizopus arrhizus | D-glucose + maltose + maltotriose | - |
? | |
| starch + H2O | quantitative analysis of reaction products of wild-type and H286 mutant enzymes, overview | Rhizopus arrhizus | D-glucose + maltose + maltotriose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ROAmy | - |
Rhizopus arrhizus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
wild-type enzyme | Rhizopus arrhizus |
| 55 | - |
mutants H286 L, H286I, H286S and H286 T | Rhizopus arrhizus |
| 60 | - |
mutant H286P | Rhizopus arrhizus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
mutant H286E | Rhizopus arrhizus |
| 5 | - |
mutants H286L, H286I, and H286D | Rhizopus arrhizus |
| 5.5 | - |
wild-type enzyme | Rhizopus arrhizus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | molecular docking of maltotriose to wild-type and mutant enzymes, overview | Rhizopus arrhizus |
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