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Literature summary for 3.2.1.1 extracted from
- Temperature adaptations in psychrophilic, mesophilic and thermophilic chloride-dependent alpha-amylases (2012), Biochimie, 94, 1943-1950.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.065 | - |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene | pH not specified in the publication, 25°C | Sus scrofa |  |
| 0.13 | - |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene | pH not specified in the publication, 25°C | Drosophila melanogaster | |
| 0.223 | - |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene | pH not specified in the publication, 25°C | Pseudoalteromonas haloplanktis | |
| 0.26 | - |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene | pH not specified in the publication, 25°C | Thermobifida fusca | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila melanogaster | P08144 | isoform Amy-p | - |
| Pseudoalteromonas haloplanktis | P29957 | - |
- |
| Sus scrofa | P00690 | isoform Amy2 | - |
| Thermobifida fusca | Q47R94 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Sus scrofa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene + H2O | - |
Sus scrofa | 4-nitrophenol + 4,6-O-ethylidene-[G7]-alpha-D-maltoheptaose | - |
? | |
| 4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene + H2O | - |
Thermobifida fusca | 4-nitrophenol + 4,6-O-ethylidene-[G7]-alpha-D-maltoheptaose | - |
? | |
| 4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene + H2O | - |
Pseudoalteromonas haloplanktis | 4-nitrophenol + 4,6-O-ethylidene-[G7]-alpha-D-maltoheptaose | - |
? | |
| 4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene + H2O | - |
Drosophila melanogaster | 4-nitrophenol + 4,6-O-ethylidene-[G7]-alpha-D-maltoheptaose | - |
? | |
| starch + H2O | - |
Sus scrofa | ? | - |
? | |
| starch + H2O | - |
Thermobifida fusca | ? | - |
? | |
| starch + H2O | - |
Pseudoalteromonas haloplanktis | ? | - |
? | |
| starch + H2O | - |
Drosophila melanogaster | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 29 | - |
- |
Pseudoalteromonas haloplanktis |
| 54 | - |
- |
Sus scrofa |
| 54 | - |
- |
Drosophila melanogaster |
| 72 | - |
- |
Thermobifida fusca |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 44.1 | - |
melting temperature | Pseudoalteromonas haloplanktis |
| 57.7 | - |
melting temperature | Drosophila melanogaster |
| 65 | - |
melting temperature | Sus scrofa |
| 74 | - |
melting temperature | Thermobifida fusca |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 153 | - |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene | pH not specified in the publication, 25°C | Thermobifida fusca | |
| 179 | - |
starch | pH not specified in the publication, 5°C | Pseudoalteromonas haloplanktis | |
| 280 | - |
starch | pH not specified in the publication, 20°C | Drosophila melanogaster | |
| 291 | - |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene | pH not specified in the publication, 25°C | Sus scrofa | |
| 364 | - |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene | pH not specified in the publication, 25°C | Drosophila melanogaster | |
| 518 | - |
starch | pH not specified in the publication, 37°C | Sus scrofa | |
| 675 | - |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene | pH not specified in the publication, 25°C | Pseudoalteromonas haloplanktis | |
| 1457 | - |
starch | pH not specified in the publication, 55°C | Thermobifida fusca | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 7.5 | - |
Sus scrofa |
| 7.2 | 7.5 | - |
Thermobifida fusca |
| 7.2 | 7.5 | - |
Pseudoalteromonas haloplanktis |
| 7.2 | 7.5 | - |
Drosophila melanogaster |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | comparison of chloride-dependent alpha-amylases from a psychrophilic Antarctic bacterium, the ectothermic fruit fly, the homeothermic pig and from a thermophilic actinomycete reveals striking continuum in the functional properties of the enzymes coupled to their structural stability and related to the thermal regime of the source organism | Sus scrofa |
| physiological function | comparison of chloride-dependent alpha-amylases from a psychrophilic Antarctic bacterium, the ectothermic fruit fly, the homeothermic pig and from a thermophilic actinomycete reveals striking continuum in the functional properties of the enzymes coupled to their structural stability and related to the thermal regime of the source organism | Thermobifida fusca |
| physiological function | comparison of chloride-dependent alpha-amylases from a psychrophilic Antarctic bacterium, the ectothermic fruit fly, the homeothermic pig and from a thermophilic actinomycete reveals striking continuum in the functional properties of the enzymes coupled to their structural stability and related to the thermal regime of the source organism | Pseudoalteromonas haloplanktis |
| physiological function | comparison of chloride-dependent alpha-amylases from a psychrophilic Antarctic bacterium, the ectothermic fruit fly, the homeothermic pig and from a thermophilic actinomycete reveals striking continuum in the functional properties of the enzymes coupled to their structural stability and related to the thermal regime of the source organism | Drosophila melanogaster |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 600 | - |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene | pH not specified in the publication, 25°C | Thermobifida fusca | |
| 2800 | - |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene | pH not specified in the publication, 25°C | Drosophila melanogaster | |
| 3000 | - |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene | pH not specified in the publication, 25°C | Pseudoalteromonas haloplanktis | |
| 4500 | - |
4-nitrophenyl alpha-D-maltoheptaoside-4,6-O-ethylidene | pH not specified in the publication, 25°C | Sus scrofa | |
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